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ProteomicsBovine Liver Membrane Preparation
We optimized an SDS depletion protocol involving KCl to precipitate the surfactant from intact proteins in the presence of urea at high pH. Provided is a comparison of 4 samples preciipitated at low pH (8) vs high pH (12), with (8 M) vs without (0 M) urea.
ORGANISM(S): Bos taurus (Bovine) 
2025-08-04 | PXD061512 | Pride
Urea Ph Sds depleted Kcl preciptation 
ProteomicsRapid and quantitative precipitation of a yeast proteome
Protein precipitation has applications as a front-end preparation strategy for proteome analysis, as well as depleting proteins in serum for small molecule analysis or for commercial preparation of bulk protein. The highly variable conditions previously implemented to precipitate proteins are reflec...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-23 | PXD015674 | Pride
Acetone Temperature Bottom-up Throughput Salt 
ProteomicsAutomated SDS depletion for mass spectrometry of membrane proteins through transmembrane electrophoresis
Membrane proteins are underrepresented in proteome analysis platforms because of their hydrophobic character, contributing to decreased solubility. Sodium dodecyl sulfate is a favored denaturant in proteomic workflows, facilitating cell lysis and protein dissolution. However, SDS impedes MS detect...
ORGANISM(S): Escherichia coli 
2016-07-07 | PXD003941 | Pride
Ltq E.coli Membrane proteins 
ProteomicsRapid trypsin digestion at elevated temperature with calcium for enhanced bottom-up proteome analysis
Trypsin is frequently employed to cleave proteins ahead of mass spectrometry characterization. Traditionally, enzyme digestion involves overnight incubation of proteins at 37 °C, which is not only time consuming but can still yield in poor digestion efficiency. While raising the temperature should t...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) Bacteria 
2023-03-11 | PXD035682 | Pride
Trypsin  calcium  bottom-up proteomics  mass spectrometry  accelerated digestion  digestion efficiency  quantitative proteomics  sample preparation 
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