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GenomicsTrypsin
Trypsin sequencing
PRJNA844483 | ENA
TranscriptomicsThe effect of trypsin-2 on human tongue squamous cell carcinoma cell line gene expression
The level of trypsin-2 has been shown to correlate with the malignancy and metastatic potential of many cancer. We used microarrays to detail how tryspin-2 overexpression changes the gene profile of human tongue squamous cell carcinoma cell line (HSC-3). We compared trypsin-2 overexpressing HSC-3 li...
ORGANISM(S): Homo sapiens 
2012-10-02 | E-GEOD-32056 | biostudies-arrayexpress
TranscriptomicsProtease activated receptor 2 (PAR2) on HEK293 activated by trypsin or 2f-LIGRLO-NH2
Human embryonic kidney cell (HEK293) were treated with PAR2 peptide agonist 2f-LIGRLO-NH2 (1.5h, 3h, 6h and 12h) or trypsin (6h). Comparison of genes similarly regulated by both treatments allowed better characterization of PAR2 induced response as both agonists had been reported as non-specific for...
ORGANISM(S): Homo sapiens 
2010-11-19 | E-GEOD-24332 | biostudies-arrayexpress
TranscriptomicsSerine protease-mediated wounding identifies localized epidermal wound response genes in Drosophila melanogaster
We have used trypsin-mediated wounding to amplify the transcriptional response to clean puncture wounding. We studied the transcriptional response of clean puncture wounding and trypsin puncture wounding in relation to unwounded wild-type late-stage embryos. Trypsin puncture wounding generally incr...
ORGANISM(S): Drosophila melanogaster 
2013-08-12 | E-MEXP-3755 | biostudies-arrayexpress
TranscriptomicsGene expression profile of gliadin effects on intestinal epithelial cells suggests novel non-enzymatic functions of pepsin and trypsin
Gliadin triggers T-cell mediated immunity in celiac disease, and has cytotoxic effects on enterocytes mediated through obscure mechanisms. In addition, gliadin transport mechanisms, potential cell surface receptors and gliadin-activated downstream signaling pathways are not completely understood. In...
ORGANISM(S): Homo sapiens 
2013-03-21 | E-GEOD-45357 | biostudies-arrayexpress
TranscriptomicsRNA-seq of human colorectal cancer HCT116 and HT29 cells by the distinct conditions of trypsin digestion
In the process of cell culture in vitro, we usually find that the cells show signs of aging, which is that the passed cells are unable to stick to the wall. Experience tells us that the time it takes for trypsin to digest the adherent cells is important. If the digestion time is too short, enough ce...
ORGANISM(S): Homo sapiens 
2024-12-21 | E-MTAB-14651 | biostudies-arrayexpress
ProteomicsExtending the coverage of Lys-C/trypsin-based bottom-up proteomics using cysteine S-aminoethylation
In this study, S-aminoethylation of Cys (AE-Cys) was carried out with 2-bromoethylamine to form pseudo-Lys, which was then digested with Lys-C or trypsin to improve proteome coverage in bottom-up proteomics. A model study with bovine serum albumin showed that the C-terminal side of Cys was successfu...
ORGANISM(S): Homo Sapiens (human) Bos Taurus 
2023-09-27 | PXD045433 | JPOST Repository
ProteomicsA method for producing heat-stable and pure trypsin
In this study we evaluated several different strategies for reductive methylation and purification of porcine trypsin in order to write a protocol that can be followed by proteomics laboratories.
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Equus caballus (Horse) 
2019-06-06 | PXD008193 | Pride
Trypsin Dimethylation Lc-ms 
OtherLysargiNase mirrors trypsin for protein C-terminal and methylation-site identification.
Data from ProteomeXchange: PXD ID: PXD001114. Experiment: LysargiNase, silac, file: LR_Ulilysin_PMA_A1_121126.mzml. Published as part of . From the Abstract: {{i}} To improve proteome coverage and protein C-terminal identification, we characterized the Methanosarcina acetivorans thermophilic protei...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32310005457 | GPMDB
Resource Reanalysis 
ProteomicsOptimized data analysis avoiding trypsin artefacts
Most bottom-up proteomics experiments share two features: The use of trypsin to digest proteins for mass spectrometry and the statistic driven matching of the measured peptide fragment spectra against protein database derived in silico generated spectra. While this extremely powerful approach in com...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-04-15 | PXD002726 | Pride
Autolysis protected trypsin Data base search 
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