Sort   by:  
 Page size 
TranscriptomicsPri-miRNA identification by generating a Drosha null/conditional-null mouse model.
We report the ability of the Drosha null/conditional-null mouse model to enable the identification of pri-miRNA transcripts. The conditional-null allele of Drosha phenocopies the null allele both in mESC and in mice, upon conversion to the null state with Cre. Examination of the effects of Drosha de...
ORGANISM(S): Mus musculus 
2015-02-09 | E-GEOD-55735 | biostudies-arrayexpress
TranscriptomicsFunctional evidence that Drosha over-expression in cervical squamous cell carcinoma affects cell phenotype and microRNA profiles [miRNA]
Comparison of miRNA expression profiles in cervical carcinoma cell lines to study the effects of Drosha expression levels Analyzed global miRNA expression profiles from 18 samples (7 samples in duplicate, 4 single sample) representing cervical carcinoma cell lines with either relative Drosha over-e...
ORGANISM(S): Homo sapiens 
2011-05-25 | E-GEOD-26175 | biostudies-arrayexpress
TranscriptomicsFunctional evidence that Drosha over-expression in cervical squamous cell carcinoma affects cell phenotype and microRNA profiles
Although gain of chromosome-5p is one of the most frequent DNA copy number imbalances in cervical squamous cell carcinoma (SCC), the genes that drive its selection remain poorly understood. In a previous cross-sectional clinical study we showed that the microRNA processor Drosha (located on chromoso...
ORGANISM(S): Homo sapiens 
2011-05-26 | E-GEOD-26177 | biostudies-arrayexpress
TranscriptomicsDiverse endonucleolytic cleavage sites in the mammalian transcriptome depend upon microRNAs, Drosha, and additional nucleases
Messenger RNAs are regulated by a variety of degradation mechanisms in mammalian cells. In the canonical animal microRNA pathway, microRNAs in complex with Argonaute proteins bind to many mRNA targets with imperfect complementarity, leading to degradation of the mRNA through the regular decay machi...
ORGANISM(S): Mus musculus 
2010-06-25 | E-GEOD-21975 | biostudies-arrayexpress
TranscriptomicsMutations in the SIX1/2 pathway and the DROSHA/DGCR8 miRNA microprocessor complex underlie high-risk blastemal type Wilms tumors
Blastemal histology in chemotherapy-treated pediatric Wilms tumors (nephroblastoma) is associated with adverse prognosis. To uncover the underlying tumor biology and find novel therapeutic leads for this subgroup of patients, we analyzed 58 blastemal-type Wilms tumors by exome and transcriptome sequ...
ORGANISM(S): Homo sapiens 
2015-02-18 | E-GEOD-60081 | biostudies-arrayexpress
ProteomicsAnalysis of the impact of DICER1 and DROSHA on the angio-genic capacity of human endothelial cells
RNAi-mediated knockdown of DICER1 and DROSHA, enzymes critically involved in miRNA biogenesis, has been postulated to affect the homeostasis and the angiogenic capacity of human endothelial cells. To re-evaluate this issue, we reduced the expression of DICER1 or DROSHA by RNAi-mediated knockdown and...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD027923 | Pride
Endothelial cells  angiogenesis  microrna biogenesis Dicer1  drosha  rna interference 
TranscriptomicsRe-evaluation of the roles of DROSHA, Exportin 5, and DICER in microRNA biogenesis
Biogenesis of canonical microRNAs (miRNAs) involves multiple steps: nuclear processing of primary miRNA (pri-miRNA) by DROSHA, nuclear export of precursor miRNA (pre-miRNA) by Exportin 5 (XPO5), and cytoplasmic processing of pre-miRNA by DICER. To gain a deeper understanding of the contribution of e...
ORGANISM(S): Homo sapiens 
2016-03-17 | E-GEOD-77989 | biostudies-arrayexpress
ProteomicsSAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA
Neural stem cell regulation is essential for the formation of the central nervous system and homeostatic neurogenesis in the adult mammalian brain. The RNAseIII Drosha, a key component of the miRNA microprocessor, plays a central role in regulating NSC maintenance partially through a miRNA-independe...
ORGANISM(S): Mus musculus (Mouse) 
2024-06-03 | PXD017677 | Pride
Neural stem cell Drosha 
TranscriptomicsRNAi knock down of four Argonaute paralogs (AGO1, AGO2, PIWI and Aubergine) and the microRNA-processing enzyme Drosha in Drosophila
RNA silencing pathways are conserved gene regulation mechanisms that lead to degradation, translational repression or transcriptional silencing of target-transcripts selected based on complementarity with small RNA molecules. The fraction of the genome regulated by these pathways has not been establ...
ORGANISM(S): Drosophila melanogaster 
2006-11-20 | E-MEXP-295 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of HeLa cells to investigate the effect of miRNA biogenesis factors on mRNA levels
This SuperSeries is composed of the following subset Series: GSE13638: Ago2 or Dicer knockdown effects on mRNA levels in HeLa GSE13639: Drosha or DGCR8 knockdown effects on mRNA levels in HeLa Refer to individual Series
ORGANISM(S): Homo sapiens 
2009-01-15 | E-GEOD-13640 | biostudies-arrayexpress
Sort   by:  
 Page size