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TranscriptomicsRecipient cell nuclear factors are required for reprogramming by nuclear transfer
Reprogramming occurs after nuclear transfer into zygotes whose genome was removed in mitosis, but not after nuclear transfer into zygotes enucleated in interphase Egli et al. Development 2010 doi:10.1242/dev.046151 Groups of 20 mouse embryos were used for the analysis. RNA amplification was done usi...
ORGANISM(S): Mus musculus 
2010-05-27 | E-GEOD-21609 | biostudies-arrayexpress
GenomicsMouse ooplasm confers context-specific reprogramming capacity
Enucleated oocytes have the remarkable ability to reprogram somatic nuclei back to totipotency. Here we investigate genome-scale DNA methylation patterns after nuclear transfer and identify specific targets for DNA demethylation as well as NT specific limitations. We find that the ooplasm can confer...
ORGANISM(S): Mus musculus 
2012-08-10 | E-GEOD-38711 | biostudies-arrayexpress
TranscriptomicsGene expression in pluripotent stem cells derived after somatic cell genome transfer into human oocytes
The exchange of the oocyte’s genome with the genome of a somatic cell, followed by the derivation of pluripotent stem cells, could enable the generation of specific cell types affected in degenerative human diseases. Such cells, carrying the patient’s genome, might be useful for cell replacement. H...
ORGANISM(S): Homo sapiens 
2011-10-05 | E-GEOD-27507 | biostudies-arrayexpress
TranscriptomicsHuman oocytes reprogram somatic cells to a pluripotent state
This SuperSeries is composed of the following subset Series: GSE27507: Gene expression in pluripotent stem cells derived after somatic cell genome transfer into human oocytes GSE28022: Gene expression in blastomeres after transfer of somatic cells into human oocytes Refer to individual Series
ORGANISM(S): Homo sapiens 
2011-10-05 | E-GEOD-28024 | biostudies-arrayexpress
TranscriptomicsHuman oocytes reprogram adult somatic cells to pluripotent stem cells [Illumina]
The stem cell lines were generated according to the principle described in Noggle et al., Nature 2011, Oct 5;478(7367):70-5. doi: 10.1038/nature10397. Title: Human oocytes reprogram somatic cells to a pluripotent state. Abstract: The exchange of the oocyte's genome with the genome of a somatic cel...
ORGANISM(S): Homo sapiens 
2014-08-12 | E-GEOD-60360 | biostudies-arrayexpress
TranscriptomicsGene expression in blastomeres after transfer of somatic cells into human oocytes
The exchange of the oocyte's genome with the genome of a somatic cell, followed by the derivation of pluripotent stem cells, could enable the generation of specific cell types affected in degenerative human diseases. Such cells, carrying the patient's genome, might be useful for cell replacement. H...
ORGANISM(S): Homo sapiens 
2011-10-05 | E-GEOD-28022 | biostudies-arrayexpress
TranscriptomicsGene expression of genetically related and unrelated human embryonic stem cell lines
Gene expression of sibling human ES cell lines are more similar to each other than unrelated cell lines. Undifferentiated human embryonic stem cells were used for analysis. We sought to determine whether genetic differences underly variations between different human ES cell lines. Sibling cell lines...
ORGANISM(S): Homo sapiens 
2009-03-19 | E-GEOD-15294 | biostudies-arrayexpress
TranscriptomicsA stem cell model of diabetes due to glucokinase deficiency
Diabetes is a disorder characterized by loss of beta cell mass and/or beta cell function, leading to deficiency of insulin relative to metabolic need. To determine whether stem cell derived-beta cells faithfully reflect the phenotypes of a diabetic subject, we generated induced pluripotent stem cell...
ORGANISM(S): Homo sapiens 
2013-04-05 | E-GEOD-45777 | biostudies-arrayexpress
TranscriptomicsReprogramming is achieved within a single cell cycle after mouse nuclear transfer
Although nuclear transfer allows the reprogramming of somatic cells to totipotency, little is known concerning the kinetics by which it takes place or the minimum requirements for its success. Here, we demonstrate that reprogramming can be achieved within a few hours and a single cell-cycle as long ...
ORGANISM(S): Mus musculus 
2011-10-03 | E-GEOD-28811 | biostudies-arrayexpress
TranscriptomicsElimination of mitochondrial DNA variants by nuclear genome transfer in human oocytes
Nuclear genome exchange between different oocytes results in efficient development and stem cell derivation 1 million cells was used for RNA extraction and labeling using the Illumina total prep RNA amplification kit. Parthenogenetic stem cell lines and embryonic stem cells
ORGANISM(S): Homo sapiens 
2012-12-01 | E-GEOD-42077 | biostudies-arrayexpress
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