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TranscriptomicsThe Cellular EJC Interactome Reveals Higher-Order mRNP Structure and an EJC-SR Protein Nexus
To uncover exon junction complex (EJC) deposition sites on cellular mRNAs, RNA footprints of EJC immuo-purified from HEK293 cells were deep sequenced. The analysis of these data revealed that major “canonical” EJC occupancy site in vivo lies 24 nucleotides upstream of exon junctions (-24 position) a...
ORGANISM(S): Homo sapiens 
2012-10-18 | E-GEOD-41154 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling of EJC depletion by RNA-Seq using the SOLiD platform (Applied Biosystems)
Signaling pathways are controlled by a vast array of post-translational mechanisms. By contrast, little is known regarding the mechanisms that regulate the expression of their core components. We conducted an RNAi screen in Drosophila for factors modulating RAS/MAPK signaling and identified the Exon...
ORGANISM(S): Drosophila melanogaster 
2010-10-19 | E-GEOD-24012 | biostudies-arrayexpress
GenomicsEJC ipaRt
EJC ipaRt: Transcriptome-wide definition of stable EJC RNA interactions in cytoplasm of adult flies
PRJEB26421 | ENA
TranscriptomicsCLIP-seq of eIF4AIII reveals transcriptome-wide mapping of the human exon junction complex
The exon junction complex (EJC) is a central effector of mRNAs fate, linking nuclear processing to mRNA transport, translation and surveillance. Little is known about its transcriptome-wide targets. We used high-throughput sequencing after crosslinking and immunoprecipitation (HITS-CLIP) in human ce...
ORGANISM(S): Homo sapiens 
2012-10-21 | E-GEOD-40778 | biostudies-arrayexpress
TranscriptomicsThe exon junction complex controls transposable element activity by ensuring the faithful splicing of the piwi transcript
The exon junction complex (EJC) is a highly conserved ribonucleoprotein complex which binds RNAs at a late stage of the splicing reaction and remains associated following export to the cytoplasm. This complex is involved in several cellular post-transcriptional processes including mRNA localization,...
ORGANISM(S): Drosophila melanogaster 
2014-09-08 | E-GEOD-57710 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of control and EJC factor (EIF4A3, RBM8A, MAGOH) knockdowns in part with EIF4A3 rescue in HeLa Tet-Off cells
The exon junction complex is involved in gene expression regulation on multiple co- and post-transcriptional levels. We aimed to investigate in HeLa Tet-Off cells which gene expression alterations can be observed upon knockdown of core EJC factors (EIF4A3, RBM8A and MAGOH) or upon EIF4A3 knockdown a...
ORGANISM(S): Homo sapiens 
2022-05-06 | E-MTAB-10771 | biostudies-arrayexpress
TranscriptomicsThe exon junction complex is required for definition and excision of neighboring introns in Drosophila
Splicing of pre-mRNAs results in the deposition of the exon junction complex (EJC) upstream of exon-exon boundaries. The EJC plays crucial post-splicing roles in export, translation, localization and nonsense-mediated decay of mRNAs. It also aids faithful splicing of pre-mRNAs containing large intro...
ORGANISM(S): Drosophila melanogaster 
2014-12-04 | E-GEOD-58830 | biostudies-arrayexpress
TranscriptomicsThe exon junction complex controls transposable element activity by ensuring faithful splicing of the piwi transcript
The exon junction complex (EJC) is a highly conserved ribonucleoprotein complex which binds RNAs during splicing and remains associated with them following export to the cytoplasm. While the role of this complex in mRNA localization, translation and degradation has been well characterized, its mecha...
ORGANISM(S): Drosophila melanogaster 
2014-08-14 | E-GEOD-59327 | biostudies-arrayexpress
TranscriptomicsmRNAseq of Drosophila S2 cells after knockdown of EJC components
This study used Drosophila melanogaster S2 cells treated with siRNA against the EJC components mago or eIF4A3, or a non-targeting control siRNA (three samples per condition). mRNA-seq libraries were prepared from the samples and the data was used to examine 'RS-exons' - exons which reconstitute a 5'...
ORGANISM(S): Drosophila melanogaster 
2018-10-15 | E-MTAB-7271 | biostudies-arrayexpress
TranscriptomicsThe exon junction complex regulates the splicing of cell polarity gene dlg1 to control Wingless signaling in development
Wingless (Wg)/Wnt signaling is conserved in all metazoan animals and plays critical roles in development. The Wg/Wnt morphogen reception is essential for signal activation, whose activity is mediated through the receptor complex and a scaffold protein Dishevelled (Dsh). We report here that the exon ...
ORGANISM(S): Drosophila melanogaster 
2016-08-19 | E-GEOD-81220 | biostudies-arrayexpress
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