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TranscriptomicsSAGE analysis of wild-type mouse brain regions
SAGE libraries generated from various brain regions of 8- to 12-week-old male C57BL/6 mice. Keywords: Micro-dissection, Brain regions, SAGE Sau3A I was used as the anchoring enzyme and Bsmf I as the tagging enzyme. Data are provided after linker removal.
ORGANISM(S): Mus musculus 
2008-02-05 | E-GEOD-7594 | biostudies-arrayexpress
TranscriptomicsLongSAGE analysis of wild-type mouse brain regions
LongSAGE libraries generated from various brain regions of 8- to 12-week-old male C57BL/6 mice. Keywords: Micro-dissection, Brain regions, SAGE Sau3A I was used as the anchoring enzyme and Mme I as the tagging enzyme. Data are provided after linker removal.
ORGANISM(S): Mus musculus 
2008-02-05 | E-GEOD-7593 | biostudies-arrayexpress
TranscriptomicsThe kidney collecting duct acid-base regulon
Kidneys are essential for acid-base homeostasis, especially when organisms cope with changes in acid or base dietary intake. Because collecting ducts constitute the final site for regulating urine acid-base balance, we undertook to identify the gene network involved in acid-base transport and regula...
ORGANISM(S): Mus musculus 
2006-07-01 | E-GEOD-4729 | biostudies-arrayexpress
TranscriptomicsEffect of potassium-depleted diet on kidney
Libraries obtained from microdissected kidney outer medullary collecting duct from C57Bl6/J mice control or after 3 days potassium-depleted diet. Keywords: other
ORGANISM(S): Mus musculus 
2004-05-30 | E-GEOD-1445 | biostudies-arrayexpress
TranscriptomicsGene expression profiles in normal and Otx2-/- early gastrulating mouse embryos
We compared gene expression profiles in wild-type and Otx2(-/-) 6.5 days postcoitum embryos by using a serial analysis of gene expression assay adapted to microdissected structures. Among a broader list, the study of six genes found to be differentially expressed allows defining a role for Otx2 in t...
ORGANISM(S): Mus musculus 
2003-09-25 | E-GEOD-683 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of monocytic leukemia cell differentiation
The human leukemia cell line U937 is a well-established model for studying monocytic cell differentiation. We used a modified protocol (SADE) of serial analysis of gene expression (SAGE) and developed a SADE linker-anchored PCR assay to investigate the pattern of expression of known genes and to ide...
ORGANISM(S): Homo sapiens 
2004-10-23 | E-GEOD-1865 | biostudies-arrayexpress
TranscriptomicsGenomic alterations in primary hyperaldosteronism
Transcriptome comparison of an aldosterone producing adenoma transcriptome and its adjacent zona glomerulosa. Both tissues are from the same individual, are differentiated for aldosterone production, but the adjacent zona glomerulosa produces no aldosterone by negative feedback. Keywords = aldostero...
ORGANISM(S): Homo sapiens 
2005-10-11 | E-GEOD-2017 | biostudies-arrayexpress
TranscriptomicsTranscriptome of a mouse kidney cortical collecting duct cell line: effects of aldosterone and vasopressin
In this study, we analyzed the transcriptome of a highly differentiated mouse clonal CCD principal cell line (mpkCCD(cl4)) and the changes in the transcriptome induced by aldosterone and vasopressin. Serial analysis of gene expression (SAGE) was performed on untreated cells and on cells treated with...
ORGANISM(S): Mus musculus 
2003-09-24 | E-GEOD-678 | biostudies-arrayexpress
TranscriptomicsSADE analysis of microdissected kidney
Libraries generated from microdissected kidney samples from the healthy pole of cancerous kidneys. Donors were from either sex. Libraries were generated using Sau3A I as anchoring enzyme. Data are provided after removal of linker derived sequences. Keywords: other
ORGANISM(S): Homo sapiens 
2003-11-13 | E-GEOD-694 | biostudies-arrayexpress
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