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ProteomicsUPS-2 - Increased Sensitivity of Mass Spectrometry by Alkaline Two-Dimensional Liquid Chromatography: Deep Cover of the Human Proteome in Gene-Centric Mode
using UPS-2 set as an example we characterized mass spectrometric approaches from the point of view of sensitivity (Sn), specificity (Sp) and accuracy (Ac)
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-11-23 | PXD007643 | Pride
Human proteome project Specificity Sensitivity Accuracy Shotgun Elected reaction monitoring Proteome 
TranscriptomicsLarge-scale temporal gene expression profiling during gonadal differentiation and early gametogenesis in rainbow-trout
Animals and samplings: Research involving animal experimentation has been approved by the author's institution (authorization number 35-14) and conforms to NIH guidelines. All-male and all-female rainbow trout populations were obtained from the INRA experimental fish farm (Drennec, France) using bre...
ORGANISM(S): Oncorhynchus mykiss 
2005-06-01 | E-GEOD-2151 | biostudies-arrayexpress
TranscriptomicsTranscription profiling to compare Nodular lymphocyte predominant Hodgkins lymphoma and T cell/histiocyte rich B cell lymphoma
T-cell/histiocyte rich B cell lymphoma (THRBL) and nodular lymphocyte predominant Hodgkin's lymphoma (NLPHL) share some morphological characteristics, including a prominent stromal reaction, but display a markedly different prognosis. To investigate the difference between the stromal reactions of th...
ORGANISM(S): Homo sapiens 
2009-12-18 | E-GEOD-7788 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of cKIT+ sorted cells from 53-137 day old fetal testes and ovaries and RNA-Seq of TRA-1-81+ H1 and UCLA1 hESCs.
We performed RNA-Seq analyses on 15 human fetal samples at 53-137 days of development, 9 female and 5 male, and identified the transcriptional changes during the transition of human cKIT+ primordial germ cells (PGCs), the precursors of gametes, to the generation of Advanced Germline Cells. Comparing...
ORGANISM(S): Homo sapiens 
2015-05-28 | E-GEOD-63392 | biostudies-arrayexpress
TranscriptomicsThe rsa3-1 mutation affects global gene expression in Arabidopsis thaliana under salt stress.
Salt stress is one of the most severe environmental conditions which cause huge losses in crop production worldwide. We identified an essential regulator of salt stress RSA3 and used the Affymetrix whole-genome arrays to study the effect of rsa3-1 mutation on global gene expression under salt stress...
ORGANISM(S): Arabidopsis thaliana 
2013-04-20 | E-GEOD-41626 | biostudies-arrayexpress
TranscriptomicsMolecular Biomarker Signature for Bladder Cancer Detection
In this study we applied differential gene expression analysis to exfoliated human urothelia obtained from patients of known bladder disease status. Selected targets from the microarray data were validated in an independent set of samples using a quantitative PCR approach. Total RNA was extracted fr...
ORGANISM(S): Homo sapiens 
2013-01-08 | E-GEOD-31189 | biostudies-arrayexpress
TranscriptomicsGene Expression Profiling Of Spinal Cord Injured Patients With Intractable Neuropathic Pain
Objective: Chronic pain is a major problem in patients with spinal cord injury (SCI). According to different studies, approximately 70% of patients with SCI experience persistent pain. Although previous gene expression profiling studies have been conducted in animal models, gene expression profiling...
ORGANISM(S): Homo sapiens 
2015-06-16 | E-GEOD-69901 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of peripheral blood mononuclear cells from patients with minimal change nephrotic syndrome
Background: Minimal change nephrotic syndrome (MCNS) is considered to be associated with T cell dysfunction, via unknown mechanisms. Experimental observations suggest that some humoral factors alter the permeability of glomerular filtration barrier. However, the nature of such factors remains still ...
ORGANISM(S): Homo sapiens 
2010-05-25 | E-GEOD-7349 | biostudies-arrayexpress
TranscriptomicsArrays of time course experiment (2-28 d) laser microdissected samples of H. pylori -infected mice
Mice were infected with Helicobacter pylori strain SS1 for 2, 7, 14 or 28 days or left uninfected. They were sacrificed at the appropriate times, the stomachs were removed and one half was plated for colony counts. The other half was embedded in OCT and cryosections were produced for 6-7 animals per...
ORGANISM(S): Mus musculus 
2012-05-11 | E-GEOD-37938 | biostudies-arrayexpress
TranscriptomicsCanonical and non-canonical EcfG sigma factors control the general stress response in Rhizobium etli
A core component of the M-NM-1-proteobacterial general stress response is the extracytoplasmic function (ECF) sigma factor EcfG, exclusively present in this taxonomic class. Half of the completed M-NM-1-proteobacterial genome sequences contain two or more copies of genes encoding M-OM-^CEcfG-like si...
ORGANISM(S): Rhizobium etli CFN 42 
2013-12-20 | E-GEOD-53517 | biostudies-arrayexpress
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