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Procyclic trypanosomes (strain 427 lister) were grown in culture under standard conditions at 27ºC in SDM79 medium with 10% foetal bovine serume (Brun and Schnenberger, 1979), in a gazed incubator (5% CO2). Logarithmically growing procyclic cells (at about 5*10^6 cells/ml, at 27°C) were added to o...
ORGANISM(S): Trypanosoma brucei 
Induction of the expression of the DHH1 DEAD:DQAD mutant from pLEW100 for 24 hours (thus, M-1 24 hours
Tetracycline). Uninduced cells were used as control.
ORGANISM(S): Trypanosoma brucei 
Induction of the expression of the DHH1 wild type from pLEW100 for 24 hours (M-1 24 hours Tetracycline). Non-induced cells were used as control.
ORGANISM(S): Trypanosoma brucei 
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