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TranscriptomicsSmall non-coding RNA expression in mouse elongated spermatids
In order to discover novel small RNAs expressed in elongated spermatids, we isolated elongated spermatids from mouse testis. The small RNA fraction (18-40nt) was cloned and sequenced from total RNA. RNAs extracted from elongated spermatids were used for high throughput sequencing analysis
ORGANISM(S): Mus musculus 
2013-01-31 | E-GEOD-40397 | biostudies-arrayexpress
ProteomicsProteome of wild type and Nsun7 knockout mice elongated spermatids
Investigation of a new RNA-modification-based mechanism in mammalian spermatogenesis. We created a mice line devoid of testis-specific m5C RNA methyltransferase Nsun7. These mice demonstrated male infertility due to a peculiar defect in sperm tail assembly – mispositioning of the longitudinal column...
ORGANISM(S): Mus musculus (Mouse) 
2026-01-12 | PXD067394 | Pride
Nsun7 Elongated spermatids Spermatogenesis Longitudinal columns Methyltransferase 
ProteomicsEvaluation of NSUN7 amount in spermatozoa, round and elongated spermatids of mice
Here we performed target proteomics of NSUN7 on sorted elongated and round spermatids, as well as mature spermatozoa. We showed that NSUN7 is present at all stages of spermiogenesis and is most abundant in round spermatids, which corresponds to the formation of flagellum and fibrous sheath assembly.
ORGANISM(S): Mus musculus (Mouse) 
2026-01-12 | PXD069148 | Pride
Nsun7 Spermatogenesis 
GenomicsSmall non-coding RNA expression in mouse elongated spermatids
In order to discover novel small RNAs expressed in elongated spermatids, we isolated elongated spermatids from mouse testis. The small RNA fraction (18-40nt) was cloned and sequenced from total RNA.
ORGANISM(S): Mus musculus 
2013-01-31 | GSE40397 | GEO
TranscriptomicsRNA Sequencing of Testicular Spermatids in Mex3d Knockout and Wild-Type Mice
Using isolated and purified round and elongated spermatids from the testes, mRNA sequencing was performed to assess the impact of MEX3D protein deficiency on RNA metabolism in spermiogenesis.
ORGANISM(S): Mus musculus 
2026-03-31 | E-MTAB-14966 | biostudies-arrayexpress
TranscriptomicsRibosome Sequencing of Testicular Spermatids in Mex3d Knockout and Wild-Type Mice
Using isolated and purified round and elongated spermatids from the testes, Ribosome sequencing was performed to assess the impact of MEX3D protein deficiency on RNA metabolism in spermiogenesis.
ORGANISM(S): Mus musculus 
2026-03-01 | E-MTAB-14988 | biostudies-arrayexpress
ProteomicsTMT-based proteomics analysis of mouse germ cells
The proteome changes were quantified in RibosomeRPL39L-/- spermatocytes and elongated spermatids using TMT 6-plex, and in spermatogonia, spermatocytes, round spermatids and elongated spermatids using TMT 10-plex by LC-MS/MS.
ORGANISM(S): Mus musculus (Mouse) 
2022-10-05 | PXD020874 | Pride
Germ cells Tmt Mouse 
GenomicsMus musculus
Small non-coding RNA expression in mouse elongated spermatids
PRJNA173873 | ENA
UnknownThe rat Tspy is preferentially expressed in elongated spermatids and interacts with the core histones.
Not available
S-EPMC1885557 | biostudies-literature
UnknownSimultaneous Purification of Round and Elongated Spermatids from Testis Tissue Using a FACS-Based DNA Ploidy Assay.
Not available
S-EPMC6590320 | biostudies-literature
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