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MetabolomicsMetabolomic analysis of mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells
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ORGANISM(S): Mus musculus 
ST000003 | MetabolomicsWorkbench
Metabolomics1H NMR metabolite monitoring during the differentiation of human induced pluripotent stem cells provides new insights into the molecular events that regulate embryonic chondrogenesis
The integration of cell metabolism with signalling pathways, transcription factor networks and epigenetic mediators is critical in coordinating molecular and cellular events during embryogenesis. Induced pluripotent stem cells (IPSCs) are an established model for embryogenesis, germ layer specificat...
2023-05-24 | MTBLS4854 | MetaboLights
MetabolomicsThe functional role of OGDH for maintaining mitochondrial respiration and identity of primed human embryonic stem cells

Human embryonic stem cells (hESCs) can self-renew infinitely or differentiate into the 3 germ layer lineages in vitro with certain cues, holding great promise to model early human embryo development ex vivo. It is wildly accepted that hESCs take advantage of both glycolysis and ...

2022-03-03 | MTBLS4223 | MetaboLights
MetabolomicsStable isotope-resolved metabolomics of WT, GYG1 KO, and GYG2 KO human embryonic stem cells using 13C-labeled glucose and palmitate
Proper regulation of glycogen metabolism is fundamental to cellular energy homeostasis, and its disruption is associated with various metabolic disorders, including glycogen storage diseases (GSDs) and potentially diabetes. Despite glycogen’s role as an essential energy reservoir, the mechanisms gov...
2025-07-15 | MTBLS12586 | MetaboLights
ProteomicsTAF4 immunoprecipitation from mouse embryonic stem cells
TAF4 directed immunoprecipitation of the the Pre-initiation complex from mouse embryonic stem cells with or without depletion of TATA-box binding protein (TBP).
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-05-27 | MSV000089562 | MassIVE
ProteomicsDiscovery of Klf2 Interactors in Mouse Embryonic Stem Cells by Immunoprecipitation-Mass Spectrometry Utilizing Exogenously Expressed Bait
Krüppel-like factor 2 (Klf2) is a DNA-binding transcription factor that regulates embryonic stem cell-specific gene expression. Transcription cofactors such as p300 acetyltransferases and Erk kinases interact with Klf2, providing an additional layer of transcription regulation in embryonic stem cell...
ORGANISM(S): Mus Musculus (mouse) 
2021-09-20 | PXD022123 | JPOST Repository
TranscriptomicsExpression & polysome association in pluripotent human embryonic stem cells
The goal was to measure the expression and polysome association of genes with polysomes in human embryonic stem cells H9 human embryonic stem cells were kept in pluripotent conditions. Total RNA and polysome extracts were isolated.
ORGANISM(S): Homo sapiens 
2012-09-01 | E-GEOD-31768 | biostudies-arrayexpress
ProteomicsComprehensive transcriptomic and proteomic characterization of human mesenchymal stem cells reveals source specific cellular markers
Mesenchymal stem cells (MSC) are multipotent cells with great potential in therapy, reflected by more than 500 MSC-based clinical trials registered with the NIH. MSC are derived from multiple tissues but require invasive harvesting and imply donor-to-donor variability. Embryonic stem cell-derived MS...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080827 | MassIVE
ProteomicsMining Missing Protein Landscape in Subcellular Proteome of Human Embryonic Stem Cells
Human embryonic stem cells (hESCs) have capacity of self-renewal and multi-lineage differentiation into derivatives of three germ layers that are of clinical importance for regeneration medicine. Despite the significant progress of hESCs study for clinical application, factors responsible for mainta...
ORGANISM(S): Homo Sapiens (human) 
2019-05-20 | PXD009840 | JPOST Repository
ProteomicsDdx39 is essential for the differentiation and telomere homeostasis of Embryonic Stem Cells
identified Ddx39 and Erk3 interacting proteins by co-immunoprecipitation and mass spectrometric analysis from Mus embryonic stem cells samples
ORGANISM(S): Mus musculus (Mouse) 
2025-11-10 | PXD048543 | Pride
Ddx39 Erk2 Embryonic stem cells Coip 
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