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Ddx39 is essential for the differentiation and telomere homeostasis of Embryonic Stem Cells

ABSTRACT: identified Ddx39 and Erk3 interacting proteins by co-immunoprecipitation and mass spectrometric analysis from Mus embryonic stem cells samples

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Embryonic Stem Cell

SUBMITTER: Shanshan Nai

LAB HEAD: lingyi chen

PROVIDER: PXD048543 | Pride | 2025-11-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	CTR_uniq.txt	Txt
	Ctr-V65-proSummary.txt	Txt
	Flag-Ddx39_uniq.txt	Txt
	T1-V65-Erk2-proSummary.txt	Txt
	checksum.txt	Txt

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Publications

Novel role for Ddx39 in differentiation and telomere length regulation of embryonic stem cells.

Nai Shanshan S Wang Meijie M Yang Jiao J Ling Bin B Dong Qiman Q Yang Xiaoqiong X Du Xiaoling X Lu Man M Liu Lin L Yu Zhongbo Z Chen Lingyi L

Cell death and differentiation 20240806 11

Erk signaling is indispensable for the self-renewal and differentiation of mouse embryonic stem cells (ESCs), as well as telomere homeostasis. But how Erk regulates these biological processes remains unclear. We identified 132 Erk2 interacting proteins by co-immunoprecipitation and mass spectrometric analysis, and focused on Ddx39 as a potential Erk2 substrate. We demonstrated that Erk2 phosphorylates Ddx39 on Y132 and Y138. Ddx39 knockout (KO) ESCs are defective in differentiation, due to reduc ...[more]

PMID: 39107495

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