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MetabolomicsMetabolic response to point mutations reveals principles of modulation of in vivo enzyme activity and phenotype
The relationship between sequence variation and phenotype is poorly understood. Here, we use metabolomic analysis to elucidate the molecular mechanism underlying the filamentous phenotype of E. coli strains that carry destabilizing mutations in dihydrofolate reductase (DHFR). We find that partial lo...
2021-06-30 | MTBLS2795 | MetaboLights
MetabolomicsAnalysis of seed germination attributes and kinetics of Polygonatum sibiricum Red. through metabolomics and transcriptomics

Polygonatum sibiricum Red. (P. sibiricum) seeds exhibit strong dormancy, which prolongs germination period, reduces germination rate, and thus hinders its production. Research on breaking seed dormancy via optimized sand storage and clarifying germination-related mechanisms is insufficient. This ...

2026-03-24 | MTBLS14022 | MetaboLights
MetabolomicsDual transcriptomic & metabolomic analysis of elicited flax sheds light on the kinetics of immune defense activation against the biotrophic pathogen Oidium lini.
Flax (Linum usitatissimum) grown under controlled conditions displayed genotype-dependent resistance to powdery mildew (Oidium lini) following COS-OGA (comprising chitosan- and pectin-derived oligomers) elicitor application. The present study reveals a two-step immune response in plant...
2025-04-25 | MTBLS8263 | MetaboLights
ProteomicsEnzyme expression kinetics by Escherichia coli during transition from rich to minimal media depends on proteome reserves
Bacterial fitness depends on adaptability to changing environments. In rich growth medium, which is replete with amino acids, Escherichia coli primarily expresses protein synthesis machineries, which comprise ~40% of cellular proteins and are required for rapid growth. Upon transition to minimal med...
ORGANISM(S): Escherichia coli 
2023-02-06 | PXD035278 | Pride
Proteomics Amino acid biosynthesis Growth transition Ribosome biogenesis Coarse-grained theory 
ProteomicsIntegrating Proteomics and Enzyme Kinetics Reveals Tissue-Specific Types of the Glycolytic and Gluconeogenic Pathways
Glycolysis is the core metabolic pathway supplying energy to cells. Whereas the vast majority of studies focus on specific aspects of the process, global analyses characterizing simultaneously all enzymes involved in the process are scarce. Here we demonstrate that quantitative label- and standard-f...
ORGANISM(S): Mus musculus (Mouse) 
2015-06-22 | PXD002156 | Pride
Gluconeogenesis Lysine acetylation Kcat -values Carbohydrate metabolism "total protein approach" Glycolysis Energy metabolism Quantitative proteomics "filter aided sample preparation" absolute protein quantitation 
ProteomicsProtein misfolding involving entanglements provides a structural explanation for the origin of stretched-exponential refolding kinetics
Stretched-exponential protein refolding kinetics, first observed decades ago, were attributed to a nonnative ensemble of structures with parallel, non-interconverting folding pathways. However, the structural origin of the large energy barriers preventing interconversion between these folding pathwa...
ORGANISM(S): Escherichia coli 
2025-03-18 | PXD053579 | Pride
Limited proteolisys mass spectrometry Stretched-exponential kinetics Refolding 
ProteomicsActive site tyrosine residues in human NQO1 homodimer are critical for non-synchronous enzyme catalysis at the two active sites
Human NQO1 is a flavoenzyme essential for the redox metabolization of many substances and associated with wide-impacting diseases such as cancer and Alzheimer´s. Recent X-ray crystallographic studies have proposed that a few residues at the active site of NQO1 (including Tyr126 and Tyr128) may contr...
ORGANISM(S): Homo sapiens (Human) 
2026-03-25 | PXD060224 | Pride
Enzyme kinetics Flavin Quinone oxidoreductase Hydride transfer Coenzyme specificity 
ModelsChance1943_Peroxidase_ES_Kinetics
Default parameter values are those in the right hand panel of Fig 12. The other panels may be obtained by setting X to 1, 2 or 4, and K3 to 0, 1/2 or 1. This model is described in: The kinetics of the enzyme-substrate compound of peroxidase. Britton Chance, Journal of Biologi...
2024-09-02 | BIOMD0000000283 | BioModels
UnknownNantenine as an acetylcholinesterase inhibitor: SAR, enzyme kinetics and molecular modeling investigations.
Not available
S-EPMC4663080 | biostudies-literature
GenomicsKinetics of denitrification at low pH by native soil microbial communities
Kinetics of denitrification at low pH by native soil microbial communities
PRJEB41993 | ENA
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