Enzyme purification

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Transcriptomics (4894)
Unknown (2128)
Proteomics (2119)
Genomics (806)
Metabolomics (19)
Methylation profiling (5)
Other (4)
Models (1)
Genomic (1)

Organisms

Homo sapiens (1357)
Mus musculus (1132)
Arabidopsis thaliana (464)
Drosophila melanogaster (295)
Saccharomyces cerevisiae (220)
Caenorhabditis elegans (206)
Schizosaccharomyces pombe (113)
Rattus norvegicus (97)
Danio rerio (75)
Escherichia coli (72)
Candida albicans (51)
Gallus gallus (41)
Escherichia coli str. K-12 substr. MG1655 (40)
Sus scrofa (33)
Zea mays (32)
Bos taurus (32)
Rhizobium leguminosarum (30)
Bacillus subtilis (28)
Staphylococcus aureus (28)
Salmo salar (27)
Mycobacterium tuberculosis (26)
Campylobacter jejuni (20)
Streptococcus pneumoniae (20)
Pseudomonas putida (19)
Apis mellifera (18)
Pseudomonas aeruginosa (16)
Hordeum vulgare (15)
Sinorhizobium meliloti (15)
Bordetella bronchiseptica (14)
Streptomyces coelicolor (14)

Organisms

Mus musculus (32)
Homo sapiens (29)
Arabidopsis thaliana (6)
Drosophila melanogaster (3)
Rattus norvegicus (3)
Saccharomyces cerevisiae (3)
Synechocystis sp. PCC 6803 (2)
Toxoplasma gondii (2)
Bos taurus (2)
Roseburia inulinivorans DSM 16841 (1)
termite metagenome (1)
Paenibacillus brasilensis (1)
Klebsiella phage P13 (1)
Streptomyces mobaraensis (1)
Cupriavidus metallidurans CH34 (1)
Komagataella pastoris (1)
Escherichia coli str. K-12 substr. MG1655 (1)
Yersinia pseudotuberculosis (1)
Lactococcus lactis subsp. lactis (1)
Carassius auratus (1)
Caenorhabditis elegans (1)
hot springs metagenome (1)
Entamoeba histolytica (1)
Carthamus tinctorius (1)
Staphylococcus aureus (1)
Gallus gallus (1)
unidentified (1)
Solanum tuberosum (1)

Repository

biostudies-arrayexpress (5569)
pride (2010)
ENA (125)
MassIVE (60)
geo (36)
jPOST (30)
MetaboLights (18)
EGA (15)
iProX (10)
NODE (3)
BioModels (1)
dbGaP (1)
GNPS (1)
GPMDB (1)
PanoramaPublic (1)
PAXDB (1)

Tissue

Cell culture (528)
Epithelial cell (168)
Permanent cell line cell (119)
Brain (77)
Leaf (61)
B cell (55)
HeLa cell (55)
Plant cell (53)
Fibroblast (51)
Blood (50)
Kidney (42)
Embryonic stem cell (41)
Liver (41)
Cell suspension culture (39)
Blood plasma (34)
T cell (31)
Heart (27)
Embryo (25)
Lung (25)
Whole body (25)
Colon (24)
Hepatocyte (21)
Blood cell (20)
Macrophage (18)
Blood serum (17)
Early embryonic cell (17)
Testis (17)
Primary cell (16)
Bone marrow (14)
Umbilical cord (2)

Disease

Breast cancer (51)
Normal (39)
Neuroblastoma (11)
Mitochondrial complex I deficiency (2)
Squamous Cell Carcinoma (2)
Porphyria NOS (2)
Not infected (2)
Cancer (2)
Mammary tumor (2)
Prostate cancer (2)
Breast carcinoma (2)
Ovarian tumor (2)
Wilms tumour (2)
XCGD (1)
HPV positive (1)
High bone mineral density (1)
Hepatomegaly (1)
Chronic lymphocytic leukemia (CLL) (1)
Unspecified peripheral T-cell lymphoma (1)
Perilobar nephrogenic rest (1)
Breast Carcinoma (1)
CUP (1)
Liver cancer (1)
Uninfected 11dpi (1)
Spindle cell tumor (1)
Serratia marcesens infected (1)
Not applicable (1)
Rett syndrome (1)
Phyllodes tumor (1)
Myocardial infarction (1)

Technology Type

Mass Spectrometry (2010)
Affinity purification coupled with mass spectrometry proteomics (1317)
Shotgun proteomics (762)
Bottom-up proteomics (217)
Gel-based experiment (156)
Data-dependent acquisition (154)
Chemical cross-linking coupled with mass spectrometry proteomics (58)
Data-independent acquisition (45)
Top-down proteomics (41)
SWATH MS (27)
SRM/MRM (22)
Mass spectrometry (17)
Affinity proteomics (12)
All-ion fragmentation (9)
Immunopeptidomics (7)
HDMSE (6)
DiaPASEF (5)
Mass spectrometry imaging (4)
MSE (3)
Metaproteomics (3)
Proteogenomics (3)
Illumina HiSeq 4000, ILLUMINA (1)
Illumina HiSeq 2000 (1)
Mass spectrometry assay (1)
PAcIFIC (1)
RNA mass spectrometry (1)

Instrument Platform

Illumina NovaSeq 6000 (187)
NextSeq 500 (134)
Illumina HiSeq 2500 (102)
Illumina HiSeq 2000 (85)
Illumina HiSeq 4000 (71)
NextSeq 550 (41)
Illumina NovaSeq X (37)
NextSeq 2000 (29)
Illumina MiSeq (29)
Scanning hardware (28)
Axon GenePix 4000B scanning hardware (27)
Axon GenePix 4000A scanning hardware (21)
GenePix 4000B [Axon Instruments] (21)
Illumina Genome Analyzer II (19)
GenePix 4000A [Axon Instruments] (18)
Axon- GenePix4000B (14)
Illumina HiSeq 1500 (14)
Illumina Genome Analyzer IIx (10)
G2565BA DNA microarray scanner [Agilent] (10)
HiSeq X Ten (9)
ScanArray 4000XL [PerkinElmer] (8)
DNBSEQ-G400 (8)
ScanArray Lite [PerkinElmer] (7)
Other (7)
ScanArray 5000 [PerkinElmer] (6)
GenePix Professional 4200A [Axon Instruments] (6)
ScanArray 4000 [PerkinElmer] (5)
Spectronic Helios Alpha UV-Vis spectrophotometer (5)
418 [Affymetrix] (5)
Vortex (5)

Publication Date

2010 (781)
2013 (632)
2014 (560)
2012 (532)
2011 (508)
2015 (496)
2025 (399)
2023 (372)
2022 (366)
2016 (360)
2024 (358)
2021 (316)
2008 (268)
2009 (266)
2020 (257)
2026 (251)
2019 (207)
2007 (199)
2018 (166)
2017 (137)
2006 (126)
2005 (106)
2004 (57)
2003 (6)
2002 (1)

UNIPROT ID/AC

1433B_HUMAN (1)
1433E_HUMAN (1)
1433F_HUMAN (1)
1433G_HUMAN (1)
1433S_HUMAN (1)
1433T_HUMAN (1)
1433Z_HUMAN (1)
2A5A_HUMAN (1)
2A5D_HUMAN (1)
2A5E_HUMAN (1)
2A5G_HUMAN (1)
2AAA_HUMAN (1)
2AAB_HUMAN (1)
2ABA_HUMAN (1)
3HIDH_HUMAN (1)
3MG_HUMAN (1)
41_HUMAN (1)
4EBP1_HUMAN (1)
4EBP2_HUMAN (1)
4F2_HUMAN (1)
5NT3A_HUMAN (1)
5NTC_HUMAN (1)
6PGD_HUMAN (1)
6PGL_HUMAN (1)
8ODP_HUMAN (1)
9606.ENSP00000233596 (1)
9606.ENSP00000291295 (1)
9606.ENSP00000299157 (1)
9606.ENSP00000325681 (1)
9606.ENSP00000348054 (1)

CHEBI ID

CHEBI:17203 (8)
CHEBI:18019 (7)
CHEBI:15971 (7)
CHEBI:16015 (7)
CHEBI:16027 (7)
CHEBI:17196 (6)
CHEBI:16467 (6)
CHEBI:16857 (6)
CHEBI:17895 (6)
CHEBI:17596 (6)
CHEBI:16828 (6)
CHEBI:16680 (5)
CHEBI:16958 (5)
CHEBI:17115 (5)
CHEBI:16335 (5)
CHEBI:17509 (5)
CHEBI:17750 (5)
CHEBI:18050 (5)
CHEBI:16704 (5)
CHEBI:17964 (5)
CHEBI:17345 (5)
CHEBI:15354 (5)
CHEBI:15603 (5)
CHEBI:16643 (5)
CHEBI:17295 (5)
CHEBI:30769 (5)
CHEBI:17768 (4)
CHEBI:33508 (4)
CHEBI:15611 (4)
CHEBI:17230 (4)

Metabolite Name

L-Lysine (4)
L-Proline (4)
L-Threonine (4)
Guanine (4)
L-Leucine (4)
L-Methionine (4)
L-Tyrosine (4)
N-Acetyl-D-glucosamine (3)
D-Glucose (3)
L-asparagine (3)
Betaine (3)
L-proline (3)
L-histidine (3)
Thiamine (3)
Pyroglutamic acid (3)
Lumichrome (3)
Putrescine (3)
L-Arginine (3)
L-Ornithine (3)
L-lysine (3)
L-Histidine (3)
Nicotinamide (3)
Choline (3)
Citric acid (3)
N-Acetylputrescine (3)
Riboflavin (3)
L-Tryptophan (3)
L-Glutamine (3)
Dodecanedioic acid (3)
Pyridoxal (3)

First Public Date

2014 (19)
2025 (18)
2021 (13)
2026 (12)
2024 (12)
2023 (11)
2022 (8)
2019 (8)
2013 (7)
2017 (5)
2018 (4)
2020 (3)
2015 (3)
2016 (2)

Modelling Approach

Other (1)

Lab affiliation

EDyP (36)
Head of the Curie Institute Mass Spectrometry Platform (29)
Analytics Core Facility Essen (ACE), Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany (14)
Head of Proteomics (10)
University of Oxford (10)
Department Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany (8)
UMR 7367 CNRS-UL IMoPA Biopôle de l'Université de Lorraine Campus Brabois-Santé 9 avenue de la forêt de Haye 54505 Vandoeuvre-les-Nancy FRANCE (7)
Core Facility for Medical Bioanalytics Proteomics Platform Tübingen (PxP) Institute for Ophthalmic Research Eberhard-Karls University of Tuebingen Elfriede-Aulhorn-Str. 7 D-72076 Tuebingen. Germany. (6)
Leibniz Institute of Plant Biochemistry, Department Biochemistry of Plant Interactions, Weinberg 3, 06120 Halle (Saale), Germany (6)
IGMM, CNRS, Univ Montpellier, Montpellier, France (5)
IGMM, CNRS, Université de Montpellier, Montpellier, France (5)
Proteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark (5)
Center for Biologics Evaluation and Research (CBER), U.S. Food and Drug Administration (FDA) (4)
Department Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany (4)
Department of Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology and BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg im Breisgau, Germany (4)
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan. (4)
ETHZ IMSB (4)
Faculty of Biology, Technion - Israel Institute of Technology (4)
Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium (4)
III. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany (4)
Moffitt Cancer Center Tampa, FL, USA (4)
Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China. (4)
The University of Melbourne (4)
Thoracic Oncology Molecular Medicine Program Moffitt Cancer Center Tampa, FL, USA (4)
University of Miami School of Medicine (4)
VIB-UGent Center for Medical Biotechnology (4)
AstraZeneca (3)
Centre de recherche du Centre Hospitalier Universitaire (CHU) de Quebec-Université Laval, Québec, QC, Canada Centre de recherche sur le cancer de l’Université Laval, Québec, QC, Canada PROTEO-Quebec Network for Research on Protein Function, Engineering, and Applications, Québec, QC, Canada Department of Molecular Biology, Medical Biochemistry and Pathology, Université Laval, Québec, QC, Canada (3)
Centre for Cancer Cell & Molecular Biology Barts Cancer Institute Queen Mary University of London Charterhouse Square London EC1M 6BQ U.K. (3)
Centre for Genomic Regulation (3)

IsPrivate

False (1)

Classification

protozoa (3)
bacteria (3)
viruses (1)

Modification

15.994915 (7)
N6 (6)
4-tetrahydrothiazine-3-carboxylic acid (5)
57.021464 (5)
2x(15)N labeled L-lysine (4)
4x(15)N labeled L-arginine (4)
-17.026549 (3)
71.037114 (3)
N6-dimethyl-L-lysine (3)
42.010565 (2)
3-dipalmitoyl-glycerol)cysteine (1)
4'-dihydroxyphenylalanine (1)
47.984744 (1)
4x(2)H labeled dimethylated L-lysine (1)
788.725776 (1)
79.966331 (1)
Not available (1)

Study type

Transcription profiling by array (2817)
RNA-seq of coding RNA (715)
ChIP-seq (537)
ChIP-chip by tiling array (240)
Comparative genomic hybridization by array (146)
Other (139)
ChIP-chip by array (117)
RNA-seq of non coding RNA (96)
Methylation profiling by high throughput sequencing (89)
Methylation profiling by array (83)
Unknown experiment type (73)
Transcription profiling by tiling array (60)
RNA-seq of coding RNA from single cells (52)
RNA-seq of total RNA (39)
ATAC-seq (36)
DNA-seq (26)
Ribo-seq (24)
Animal - High-throughput sequencing (22)
Cell line - High-throughput sequencing (19)
High-throughput sequencing (18)
Genotyping by array (15)
RIP-seq (13)
Single nucleus RNA sequencing (13)
Transcription profiling by RT-PCR (12)
MicroRNA profiling by high throughput sequencing (9)
4C (9)
Transcription profiling by SAGE (8)
Human - High-throughput sequencing (8)
CLIP-seq (8)
Bisulfite-seq (8)

Release Date

2010 (833)
2013 (711)
2014 (635)
2012 (597)
2011 (577)
2015 (559)
2016 (410)
2008 (316)
2009 (305)
2021 (282)
2025 (261)
2022 (249)
2007 (229)
2024 (204)
2023 (204)
2020 (199)
2019 (173)
2018 (164)
2006 (156)
2017 (151)
2026 (139)
2005 (136)
2004 (72)
1999 (26)
2003 (23)
2001 (21)
2000 (20)
1998 (17)
2002 (15)
1997 (2)

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A native chromatin purification system for epigenomic profiling in C. elegans

We describe an in vivo chromatin purification system for genome-wide epigenetic profiling in C. elegans. In this system, we coexpressed the E. coli biotin ligase enzyme (BirA), together with the C. elegans H3.3 gene fused to BioTag, a 23-amino acid peptide serving as a biotinylation substrate for Bi...

ORGANISM(S): Caenorhabditis elegans

2010-05-19 | E-GEOD-18898 | biostudies-arrayexpress

Expression and purification of the membrane enzyme selenoprotein K.

Not available

S-EPMC3483405 | biostudies-literature

DRUSP_A novel method of super-enhanced purification of ubiquitinome

A novel approach that combines Denatured-Refolded Ubiquitinated Sample Preparation (DRUSP) with a tandem hybrid specific UBD (ThUBD) for ubiquitinomic analysis.

ORGANISM(S): Mus Musculus

PXD055428 |

The Extracellular RNA Quality Control (exRNAQC) study: Small RNA sequencing of RNA purification kit study (exRNAQC011)

To evaluate the impact of the RNA purification method on extracellular RNA (exRNA) sequencing, 8 different RNA purification kits were compared by applying Small RNA sequencing (Illumina) to exRNA from human healthy donor plasma. Minimum and maximum plasma input volumes recommended by the manufacture...

ORGANISM(S): Homo sapiens

2021-06-10 | E-MTAB-10505 | biostudies-arrayexpress

Environmental Assessment of Enzyme Production and Purification.

Not available

S-EPMC7865607 | biostudies-literature

A new thermoactive pullulanase from Desulfurococcus mucosus: cloning, sequencing, purification, and characterization of the recombinant enzyme after expression in Bacillus subtilis.

Not available

S-EPMC94778 | biostudies-literature

N-terminal fusion of the N-terminal domain of bacterial enzyme I facilitates recombinant expression and purification of the human RNA demethylases FTO and Alkbh5.

Not available

S-EPMC6942671 | biostudies-literature

Cloning and expression of Saccharomyces cerevisiae SUC2 gene in yeast platform and characterization of recombinant enzyme biochemical properties.