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GenomicsChIP-Seq of human MCF-7 cells with ER-alpha and ER-beta anitbodies following estrogen treatment
We performed chromatin immunoprecipitation (ChIP) with overnight with antibodies against the C-terminus (HC-20, from Santa Cruz Biotechnology, Europe) or the N-terminus (anti-Estrogen Receptor 18-32, from SigmaAldrich, Italy) of human ER-alpha with or without estrogen treatment for 45 minutes on TAP...
ORGANISM(S): Homo sapiens 
2010-12-01 | E-MTAB-345 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human cancer cell line MCF-7 - estrogen effects where MCF7 is co-expressing ERa and ERb
Two subtypes of the estrogen receptor, ERalpha and ERbeta, mediate the actions of estrogens, and the majority of human breast tumors contain both ERalpha and ERbeta. To examine the possible interactions and modulatory effects of ERbeta on ERalpha activity, we have used adenoviral gene delivery to pr...
ORGANISM(S): Homo sapiens 
2009-04-24 | E-GEOD-4006 | biostudies-arrayexpress
TranscriptomicsTranscriptional effect of CRH on MCF7 estrogen receptor signalling
MCF7 cells were stimulated with vehicle or 100nM corticotropin releasing hormone (CRH) for 24h. The effect of CRH on the expression of genes relevant to estrogen signalling was investigated by using the Human Estrogen Receptor Signaling RTM-BM-2 ProfilerM-bM-^DM-" PCR Array (SABioscience Corp). qPCR...
ORGANISM(S): Homo sapiens 
2013-07-02 | E-GEOD-47958 | biostudies-arrayexpress
TranscriptomicsFoxA1 is a critical determinant of Estrogen Receptor function and endocrine response
This SuperSeries is composed of the following subset Series: GSE25314: FoxA1 is a critical determinant of Estrogen Receptor function and endocrine response (part I) GSE25315: FoxA1 is a critical determinant of Estrogen Receptor function and endocrine response (part II) Refer to individual Series
ORGANISM(S): Homo sapiens 
2010-11-17 | E-GEOD-25316 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of rat peripheral blood leukocytes treated with the estrogen receptor agonists estradiol, PPT and DPN to investigates the functional roles of estrogen receptor alpha and beta
This experiment investigates the functional roles of estrogen receptor alpha and beta in peripheral blood leukocytes by using selective estrogen receptor agonists. The agonists that are used are estradiol (E2), the selective ER-alpha agonist PPT (4,4',4''-(4-Propyl-[1H]-pyrazole-1,3,5-triyl)trisphen...
ORGANISM(S): Rattus norvegicus 
2007-05-30 | E-TABM-269 | biostudies-arrayexpress
GenomicsTLE1 mediates estrogen receptor binding
Estrogen receptor (ER) binds to distal enhancers within the genome and requires additional factors, such as the Forkhead protein FoxA1, for mediating chromatin interactions. We now show that the human Groucho protein, Transducin-like enhancer protein 1 (TLE1), positively assists some ER-chromatin in...
ORGANISM(S): Homo sapiens 
2012-11-14 | E-MTAB-1368 | biostudies-arrayexpress
TranscriptomicsCharacterization of macrophage - cancer cell crosstalk in estrogen receptor positive and triple-negative breast cancer
We performed whole transcriptome sequencing of human monocytes that were co-cultured with estrogen receptor positive (ER+) or triple-negative (TNBC) breast cancer cell lines and studied the biological responses related to the differential gene activation in both cell types to understand how differen...
ORGANISM(S): Homo sapiens 
2015-11-19 | E-GEOD-75130 | biostudies-arrayexpress
TranscriptomicsEstrogen receptor prevents p53-dependent apoptosis in breast cancer
More than two thirds of breast cancers express the estrogen receptor (ER) and depend on estrogen for growth and survival. Therapies targeting ER function including aromatase inhibitors that block the production of estrogens and ER antagonists that alter ER transcriptional activity play a central rol...
ORGANISM(S): Homo sapiens 
2012-11-02 | E-GEOD-39870 | biostudies-arrayexpress
TranscriptomicsRapid estrogen receptor signaling is essential for the protective effects of estrogen against vascular injury
Background: Clinical trial and epidemiological data support that the cardiovascular effects of estrogen are complex, including a mixture of both potentially beneficial and harmful effects. In animal models, estrogen protects females from vascular injury and inhibits atherosclerosis. These effects ar...
ORGANISM(S): Mus musculus 
2012-09-01 | E-GEOD-35558 | biostudies-arrayexpress
GenomicsH4K12ac is regulated by estrogen receptor-alpha and is associated with BRD4 function and inducible transcription
Hormone-dependent gene expression requires dynamic and coordinated epigenetic changes. Estrogen receptor-positive (ER+) breast cancer is particularly dependent upon extensive chromatin remodeling and changes in histone modifications for the induction of hormone-responsive gene expression. Our previo...
ORGANISM(S): Homo sapiens 
2015-05-02 | E-GEOD-65886 | biostudies-arrayexpress
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