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GenomicsChIP-Seq of human MCF-7 cells with ER-alpha and ER-beta anitbodies following estrogen treatment
We performed chromatin immunoprecipitation (ChIP) with overnight with antibodies against the C-terminus (HC-20, from Santa Cruz Biotechnology, Europe) or the N-terminus (anti-Estrogen Receptor 18-32, from SigmaAldrich, Italy) of human ER-alpha with or without estrogen treatment for 45 minutes on TAP...
ORGANISM(S): Homo sapiens 
2010-12-01 | E-MTAB-345 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of human MCF-7 cells with ER-beta tagged with TAP-tag at the C-term or N-term or expressing ER-alpha tagged
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag respectively at the C-term and at the N-term (C-TAP-ER-beta and N-TAP-ER-beta) or expressing ER-alpha tagged (C-TAP-ER-alpha). All were grown in Dulbecco's modified Eagle's medium (DMEM). Then c...
ORGANISM(S): Homo sapiens 
2011-10-11 | E-TABM-1052 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of BRCA1 wild type and mutant human breast tumors to examine expression of ER-alpha
Looking at the expression of ER alpha in BRCA1 and mutant tumours
ORGANISM(S): Homo sapiens 
2007-11-22 | E-TABM-366 | biostudies-arrayexpress
TranscriptomicsRNA-seq of human HepG2 cells treated with sodium oleate, 17β-estradiol and ER-alpha siRNA
HepG2 cells are only treated with 60 μg/ml sodium oleate as group 1, and treated with 60 μg/ml sodium oleate and 50 ng/ml E2 as group 2, and treated with 60 μg/ml sodium oleate, 50 ng/ml E2 and ER-alpha as group 3. All the treatment last for 48 hours and each group have two replications.
ORGANISM(S): Homo sapiens 
2019-10-05 | E-MTAB-7318 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human MCF7 cells following short-term E deprivation, ER-alpha siRNA knockdown and oxidant treatments
To investigate the oxidant sensitivity of E/ER regulated gene expression, E/ER regulated genes are identified using E deprivation or; ER-alpha siRNA knockdown; and oxidative stress responsive is determined by 8hr exposure to diamide, hydrogen peroxide and menadione Experiment Overall Design: MCF7 ce...
ORGANISM(S): Homo sapiens 
2008-11-13 | E-GEOD-10061 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of human MCF-7 breast cell clones expressing ER-beta tagged with TAP-tag at the C-terminal or N-terminal
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag respectively at the C-term and at the N-term (C-TAP-ER-beta and N-TAP-ER-beta) or expressing ER-alpha tagged (C-TAP-ER-alpha) as previously described.Cells were cultured in standard growth condit...
ORGANISM(S): Homo sapiens 
2011-10-11 | E-TABM-1053 | biostudies-arrayexpress
TranscriptomicsGenome wide expression analysis of ER alpha and beta with or without E2 treatment in U2OS cell line.
Estrogen receptorï?  beta (ER beta) has potent anti-proliferative and anti-inflammatory properties, suggesting that ER beta-selective agonists might be a new class of therapeutic and chemopreventative agents. To understand how ER beta regulates genes, we identified genes regulated by the unliganded ...
ORGANISM(S): Homo sapiens 
2010-05-28 | E-GEOD-21769 | biostudies-arrayexpress
no omics definedMicroRNA profiling by array of human MCF-7 breast cancer cells with ER-beta tagged at the C-terminal or N-terminal and treated with 17-beta-estradiol
Human estrogen-responsive breast cancer cell line MCF-7 TET Off (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag respectively at the C-term and at the N-term (C-TAP-ER-beta and N-TAP-ER-beta) or expressing ER-alpha tagged (C-TAP-ER-alpha) as previously described.(...
ORGANISM(S): Homo sapiens 
2011-07-29 | E-TABM-1055 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of rat peripheral blood leukocytes treated with the estrogen receptor agonists estradiol, PPT and DPN to investigates the functional roles of estrogen receptor alpha and beta
This experiment investigates the functional roles of estrogen receptor alpha and beta in peripheral blood leukocytes by using selective estrogen receptor agonists. The agonists that are used are estradiol (E2), the selective ER-alpha agonist PPT (4,4',4''-(4-Propyl-[1H]-pyrazole-1,3,5-triyl)trisphen...
ORGANISM(S): Rattus norvegicus 
2007-05-30 | E-TABM-269 | biostudies-arrayexpress
GenomicsInvestigation of ER alpha Pvu2 genetic variants
An investigation to confirm the relationships of ER alpha Pvu2 polymorphisms to osteoporosis.
ORGANISM(S): Homo Sapiens 
2023-04-05 | PRJEB60949 | EVA
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