Ergic - OmicsDI

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Single-Cell Extraction: For enzymatic digestion, pronase (Septomyces argeus, 1 mg/mL) was applied for 25 minutes at room temperature for P0 to P8 datasets. Cells were dissociated and triturated into a single-cell suspension in ACSF supplemented with 1% fetal calf serum (FCS) and DNase (1 µL/10 mL). Trituration was performed using three glass Pasteur pipettes of decreasing diameters. For P30 datasets, enzymatic digestion and cell dissociation were carried out using the Worthington Papain Dissociation System, following the manufacturer’s instructions. Single-Nuclei Extraction: For nuclei isolation, the Hibernate™-E Medium was removed, and 500 µL of chilled 0.1X NP40 Lysis Buffer was added. Tissue was homogenized using a Pellet Pestle (15 strokes) and incubated on ice for 5 minutes. The suspension was pipette-mixed 10 times using a wide-bore pipette tip and incubated for an additional 10 minutes on ice. Following lysis, 500 µL of chilled wash buffer was added, and the mixture was pipette-mixed 5 times using a regular-bore pipette tip. The suspension was filtered through a 30 µm cell strainer into a 50 mL tube to remove debris. The filtered suspension was transferred to a 1.5 mL tube and centrifuged at 950 × g for 10 minutes at 4°C. The supernatant was carefully removed, and the nuclei pellet was retained for downstream analysis. (2)
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Center for Diagbostik, Institute of Neuropathology, University Medical Center Hamburg Eppendorf, Germany (1)
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Shanghai Center for Systems Biomedicine Shanghai Jiaotong University B207, Bldg for Systems Biomedicine 800 Dongchuan Rd Shanghai, China, 200240 (1)
University of Arkansas for Medical Sciences, Little Rock, AR, 72210 (1)
Université Paris Cité/CNRS, Institut Jacques Monod, FRANCE (1)

Tags

xref:PubMed:31353184 (2)
xref:PubMed:42247288 (2)
xref:PubMed:18331641 (1)
xref:PubMed:42121876 (1)
xref:PubMed:40931074 (1)
xref:PubMed:18927111 (1)
xref:PubMed:39378153 (1)

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SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.

Not available

S-EPMC8891076 | biostudies-literature

A comparison of GABA-ergic (propofol) and non-GABA-ergic (dexmedetomidine) sedation on visual and motor cortical oscillations, using magnetoencephalography.

Not available

S-EPMC9227747 | biostudies-literature

Identification of ERGIC-53 as an intracellular transport receptor of alpha1-antitrypsin.

Not available

S-EPMC2265576 | biostudies-literature

Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport.

Not available

S-EPMC10944485 | biostudies-literature

TUG protein acts through a disordered region to organize the early secretory pathway

The Endoplasmic Reticulum (ER)-Golgi Intermediate Compartment (ERGIC) is a network of tubules and vesicles known for producing COPI vesicles and receiving COPII vesicles from the ER. Much about its identity, stability, and regulation remains unknown. Here, we show that the TUG (UBXN9, Aspscr1) prote...

ORGANISM(S): Mus musculus (Mouse)

2025-06-10 | PXD064240 | Pride