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TranscriptomicsGenes affected by Ret activation during estrogen stimulation or inhibition in MCF7/Aro cells
Endocrine therapy is the main therapeutic option for patients with estrogen receptor alpha positive (ER+) breast cancer. Nevertheless, most of them become estrogen-independent and relapse after the treatment. Ret is a tyrosine kinase receptor that shows elevated expression levels in ER+ human breast...
ORGANISM(S): Homo sapiens 
2013-09-25 | E-GEOD-41405 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human cancer cell line MCF-7 - estrogen effects where MCF7 is co-expressing ERa and ERb
Two subtypes of the estrogen receptor, ERalpha and ERbeta, mediate the actions of estrogens, and the majority of human breast tumors contain both ERalpha and ERbeta. To examine the possible interactions and modulatory effects of ERbeta on ERalpha activity, we have used adenoviral gene delivery to pr...
ORGANISM(S): Homo sapiens 
2009-04-24 | E-GEOD-4006 | biostudies-arrayexpress
GenomicsFunctional Importance of eRNAs for Estrogen-dependent Gene Transcriptional Activation
The functional importance of gene enhancers in regulated gene expression is well established. In addition to widespread transcription of long non-coding RNA (ncRNA) transcripts in mammalian cells, bidirectional ncRNAs referred to as eRNAs are present on enhancers. However, it has remained unclear wh...
ORGANISM(S): Homo sapiens 
2013-06-04 | E-GEOD-45822 | biostudies-arrayexpress
Transcriptomics17beta-estradiol (E2)-regulated gene expression across time in estrogen-dependent and estrogen deprivation-resistant MCF-7-derived breast cancer cell lines.
A series of MCF-7 variants were previously developed that are either estrogen-dependent for growth (MCF-7:WS8 cells), or resistant to estrogen deprivation and refractory (MCF-7:2A) or sensitive (MCF-7:5C) to E2-induced apoptosis. To identify genes associated with E2-induced apoptosis, estrogen depri...
ORGANISM(S): Homo sapiens 
2011-11-21 | E-GEOD-29917 | biostudies-arrayexpress
TranscriptomicsCharacterization of macrophage - cancer cell crosstalk in estrogen receptor positive and triple-negative breast cancer
We performed whole transcriptome sequencing of human monocytes that were co-cultured with estrogen receptor positive (ER+) or triple-negative (TNBC) breast cancer cell lines and studied the biological responses related to the differential gene activation in both cell types to understand how differen...
ORGANISM(S): Homo sapiens 
2015-11-19 | E-GEOD-75130 | biostudies-arrayexpress
GenomicsChIP-Seq of human MCF-7 cells with ER-alpha and ER-beta anitbodies following estrogen treatment
We performed chromatin immunoprecipitation (ChIP) with overnight with antibodies against the C-terminus (HC-20, from Santa Cruz Biotechnology, Europe) or the N-terminus (anti-Estrogen Receptor 18-32, from SigmaAldrich, Italy) of human ER-alpha with or without estrogen treatment for 45 minutes on TAP...
ORGANISM(S): Homo sapiens 
2010-12-01 | E-MTAB-345 | biostudies-arrayexpress
no omics definedTranscription profiling of breast cancer cells with TET-OFF regulated ERb expression treated with estradiol or ICI, an estrogen receptor antagonist
Assess the full impact of estrogen receptor beta on transcription by a full transcriptome analysis of ERa and ERb-mediated gene regulation in T47D breast cancer cell line with TET-OFF regulated ERb expression.
ORGANISM(S): Homo sapiens 
2007-12-17 | E-MEXP-969 | biostudies-arrayexpress
TranscriptomicsTranscriptional effect of CRH on MCF7 estrogen receptor signalling
MCF7 cells were stimulated with vehicle or 100nM corticotropin releasing hormone (CRH) for 24h. The effect of CRH on the expression of genes relevant to estrogen signalling was investigated by using the Human Estrogen Receptor Signaling RTM-BM-2 ProfilerM-bM-^DM-" PCR Array (SABioscience Corp). qPCR...
ORGANISM(S): Homo sapiens 
2013-07-02 | E-GEOD-47958 | biostudies-arrayexpress
TranscriptomicsBreast cancer cells resistant to hormone deprivation maintain an estrogen receptor alpha-dependent, E2F-directed transcriptional program
A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. To model resistance to aromatase inhibitor (AI) therapy, long-term estrogen-deprived (LTED) derivatives of MCF-7 and HCC-1428 cells were generated through culture for 3 and 7 months under hormone...
ORGANISM(S): Homo sapiens 
2012-05-13 | E-GEOD-22533 | biostudies-arrayexpress
GenomicsExogenous Estrogen Enhances T Cell Activation in Male Primates
Exogenous Estrogen Enhances T Cell Activation in Male Primates
PRJNA1284950 | ENA
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