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ProteomicsIdentification of ETV6 bound protein by affinity purification of ETV6-HA fusion protein in REH cells
To determine the protein partners of ETV6, we expressed ETV6 and HA-tagged ETV6 in Reh pre-B leukemic cells. Anti-HA magnetic beads were used for affinity purification of ETV6-HA (and ETV6 control) from the nuclear and cytoplasmic fraction. Following purification on-beads digest and LC-MS/MS experim...
ORGANISM(S): Homo sapiens (Human) 
2022-01-26 | PXD031001 | Pride
Leukemia All Etv6 
GenomicsChIP-seq in the cell line NALM-6 for RUNX1 and ETV6-RUNX1 to assess competition between the two proteins for DNA binding
RUNX1 and ETV6-RUNX1 possess the same DNA-binding runt domain and are therefore expected to bind to canonical RUNX motifs. As the ETV6-RUNX1 fusion arises in the context of native RUNX1 expression, and since RUNX1 is retained or amplified in B-ALL, the two proteins are likely to compete for the same...
ORGANISM(S): Homo sapiens 
2022-10-31 | E-MTAB-12209 | biostudies-arrayexpress
GenomicsHistone H3, lysine 27 acetylation ChIP-seq in NALM6 expressing ETV6-RUNX1
ETV6-RUNX1 is a fusion protein bringing together almost the entire coding sequence of RUNX1, including the DNA binding runt domain, and the N-terminus of ETV6 which is known to include transcriptional repressors including histone deacetylation 3 (HDAC3). Here we perform ChIP-seq for the active chrom...
ORGANISM(S): Homo sapiens 
2022-10-31 | E-MTAB-12207 | biostudies-arrayexpress
GenomicsChIP-seq for RUNX1 and ETV6-RUNX1 in an induced pluripotent stem cell model of ETV6-RUNX1 pre-leukaemia
ETV6-RUNX1 is a first-hit mutation in childhood B cell precursor acute lymphoblastic leukaemia. ETV6-RUNX1 is a fusion protein which inherits the DNA-binding runt domain from RUNX1. Here we performed chromatin precipitation for native RUNX1 and ETV6-RUNX1 using RUNX1 antibodies and specifically for ...
ORGANISM(S): Homo sapiens 
2022-10-31 | E-MTAB-12208 | biostudies-arrayexpress
TranscriptomicsChIP-seq for RUNX1 and ETV6-RUNX1 in B-ALL cell lines and patient samples
Identification of RUNX1 and ETV6-RUNX1 binding sites
ORGANISM(S): Homo sapiens 
2022-04-10 | E-MTAB-10312 | biostudies-arrayexpress
TranscriptomicsRNA-seq as a global measure of the similarity between human pluripotent stem cell and fetal liver derived B cell hierarchies
Evidence suggests childhood acute lymphoblastic leukemia (cALL) arises in early human development. Existing models of pre-leukemic initiation using the ETV6-RUNX1 fusion do not recapitulate human disease, highlighting the need for a developmentally relevant human model system. A human pluripotent st...
ORGANISM(S): Homo sapiens 
2018-02-08 | E-MTAB-6382 | biostudies-arrayexpress
GenomicsETV6-NCOA2 expression profile in T/M mixed-phenotype acute leukemia [ETV6-NCOA2 human]
ETV6-NCOA2 expression profile in T/M mixed-phenotype acute leukemia [ETV6-NCOA2 human]
PRJNA679212 | ENA
TranscriptomicsAffy SNP-array data for 23 ETV6/RUNX1-positive ALL patient samples and one cell line
The ETV6/RUNX1 fusion gene, present in 25% of B-lineage childhood acute lymphoblastic leukemia (ALL), is thought to represent an initiating event, which requires additional genetic changes for leukemia development. To identify additional genetic alterations, 24 ETV6/RUNX1-positive ALLs were analyzed...
ORGANISM(S): Homo sapiens 
2010-07-07 | E-GEOD-19996 | biostudies-arrayexpress
TranscriptomicsGene Expression Analyisis of T-ALL LOUCY cell lines treated with ETV6 siRNA vs. scramble control siRNA
Transcriptional effects of ETV6 inactivation in immature T-ALL were investigated by gene expression analysis upon siRNA-mediated knockdown of ETV6 in LOUCY cells, a T-ALL cell line with a transcriptional program highly related to that of immature T-ALLs. Gene Set Enrichment Analysis (GSEA) of the ET...
ORGANISM(S): Homo sapiens 
2011-11-24 | E-GEOD-33820 | biostudies-arrayexpress
TranscriptomicsTEL-AML1 (ETV6-RUNX1) in B-cells and leukemia (part 4)
We identified directly and indirectly regulated target genes utilizing an inducible TEL-AML1 system derived from the murine pro B-cell line BA/F3 and a monoclonal antibody directed against TEL-AML1. By integration of promoter binding identified with ChIP-on-chip, gene expression and protein output t...
ORGANISM(S): Homo sapiens 
2013-11-04 | E-GEOD-50733 | biostudies-arrayexpress
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