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ProteomicsHeat inactivation of foetal bovine serum performed after EV-depletion influences the proteome of cell-derived extracellular vesicles
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be influenced by cell culture conditions such as the presence of foetal bovine serum (FBS). Although several studies have evaluated the effect of removing FBS-derived EVs by ultracentrifugation (UC), le...
ORGANISM(S): Bos taurus (Bovine) 
2024-01-26 | PXD047505 | Pride
Ev depletion by ultracentrifugation Foetal bovine serum (fbs) Extracellular vesicles Fbs heat-inactivation 
UnknownHeat inactivation of foetal bovine serum performed after EV-depletion influences the proteome of cell-derived extracellular vesicles.
Not available
S-EPMC10805629 | biostudies-literature
UnknownFunctional repertoire of EV-associated miRNA profiles after lipoprotein depletion via ultracentrifugation and size exclusion chromatography from autologous blood products.
Not available
S-EPMC7955123 | biostudies-literature
UnknownImproving the Purity of Extracellular Vesicles by Removal of Lipoproteins from Size Exclusion Chromatography- and Ultracentrifugation-Processed Samples Using Glycosaminoglycan-Functionalized Magnetic Beads.
Not available
S-EPMC11367580 | biostudies-literature
UnknownSalting-Out Approach Is Worthy of Comparison with Ultracentrifugation for Extracellular Vesicle Isolation from Tumor and Healthy Models.
Not available
S-EPMC8699204 | biostudies-literature
UnknownEfficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serum.
Not available
S-EPMC5795649 | biostudies-literature
GenomicsQuestioning the purity of the media - Extracellular small non-coding RNA contaminants in fetal bovine serum and serum-free media
In the research field of extracellular vesicles (EVs), the use of EV-depleted fetal bovine serum (FBS) for in vitro studies is highly recommended to eliminate the confounding effects of media derived EVs. EV-depleted FBS may either be prepared by ultracentrifugation or bought commercially, neverthel...
ORGANISM(S): other sequences Bos taurus 
2019-04-30 | GSE120594 | GEO
TranscriptomicsSerum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture
Extracellular vesicles (EVs) are involved in intercellular communication in health and disease and affect processes including immune and antiviral responses. We have previously demonstrated that ultracentrifuged serum is depleted of EVs and, when used in cell culture media, is associated with declin...
ORGANISM(S): Homo sapiens 
2016-10-23 | GSE89067 | GEO
UnknownDepletion of soluble cytokines unlocks the immunomodulatory bioactivity of extracellular vesicles.
Not available
S-EPMC10405237 | biostudies-literature
TranscriptomicsComparative transcriptomic analysis of human and Drosophila extracellular vesicles reveals extensive conservation
Extracellular vesicles (EVs) are membrane-enclosed nanoparticles containing specific repertoires of genetic material. In mammals, EVs can mediate the horizontal transfer of various cargos and signaling molecules, notably miRNA and mRNA species. Whether this form of intercellular communication prevai...
ORGANISM(S): Homo sapiens 
2016-06-15 | E-GEOD-76173 | biostudies-arrayexpress
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