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TranscriptomicsA Basic Domain in the Histone H2B N-terminal Tail is Important for Nucleosome Assembly by FACT
Nucleosome assembly in vivo requires assembly factors, such as histone chaperones, to bind to histones and mediate their deposition onto DNA. In yeast, the essential histone chaperone FACT (FAcilitates Chromatin Transcription) functions in nucleosome assembly and H2A-H2B deposition during transcript...
ORGANISM(S): Saccharomyces cerevisiae 
2016-08-10 | E-GEOD-80831 | biostudies-arrayexpress
TranscriptomicsFACT and Spt6 Prevent Cryptic Transcription by Impeding H2A.Z Loading in Gene Bodies
H2A.Z is a highly conserved histone variant involved in several key nuclear processes. It is incorporated into promoters by SWR-C-related chromatin remodeling complexes, but whether it is also actively excluded from non-promoter regions is not clear. Here, we provide genomic and biochemical evidence...
ORGANISM(S): Saccharomyces cerevisiae 
2015-05-12 | E-GEOD-62880 | biostudies-arrayexpress
ProteomicsFACT, PAF-C, and CKII
Protein-protein interaction analysis for RNA Polymerase II and its elongation factors including: the PAF Complex, the FACT Complex, casein kinase II, DSIF, and TFIIF. These studies also characterized a number of casein kinase II phosphorylation sites on the subunits of the PAF Complex.
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2015-07-20 | MSV000079197 | MassIVE
ProteomicsPyruvate facilitates FACT-mediated γH2AX loading to chromatin and promotes the radiation resistance of glioblastoma
DNA repair confers the resistance of tumor cells to DNA-damaging anticancer therapies, while how reprogrammed metabolism in tumor cells contributes to such process remains poorly understood. Pyruvate kinase M2 isoform (PKM2) catalyzes the conversion of phosphoenolpyruvate to pyruvate and regulates t...
ORGANISM(S): Homo Sapiens Escherichia Coli 
2021-11-18 | PXD029790 |
ProteomicsN-terminus of Spt16 anchors FACT to Mcm2 for parental histone recycling
Parental histone recycling is essential for the restoration of chromatin-based epigenetic information during chromatin replication; however, the specific mechanisms underlying the local recycling of parental histones remain poorly understood. Here, we reveal an unexpected role of the Spt16-N domain ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-01-26 | PXD043392 | Pride
Mcm2-7 Fact，parental histone recycling 
GenomicsFACT mediates cohesin function on chromatin
FACT mediates cohesin function on chromatin
PRJNA485963 | ENA
ProteomicsPhosphorylation of the FACT histone chaperone subunit SPT16 modulates Arabidopsis development and affects chromatin at RNA polymerase II transcriptional start sites
The heterodimeric histone chaperone FACT consisting of SSRP1 and SPT16 contributes to dynamic nucleosome rearrangements during various DNA-dependent processes including transcription. In search of post-translational modifications that may regulate the activity of FACT, SSRP1 and SPT16 were isolated ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-06-09 | PXD028534 | Pride
Arabidopsis Fact complex Acetylation Phosphorylation 
TranscriptomicsThe yeast and human FACT complexes resolve R-loop-mediated transcription-replication conflicts
Transcription is a major obstacle for replication fork progression and transcription-replication collisions constitute a main cause of genome instability. At a genome-wide scale these obstacles can be detected by the accumulation of the replicative Rrm3 helicase required for RF progression through p...
ORGANISM(S): Saccharomyces cerevisiae 
2014-03-17 | E-GEOD-51653 | biostudies-arrayexpress
GenomicsFACT orchestrates the interplay between chromatin structure and transcription
FACT orchestrates the interplay between chromatin structure and transcription
PRJNA1008118 | ENA
TranscriptomicsExpression data from Saccharomyces cerevisiae strains carrying the spt16-11 or the pob3-7 allele
The conserved FACT (FAcilitates Chromatin Transcription) complex is a chromatin-reorganizing complex that promotes RNAPII transcription through chromatin templates by interacting with histones. It facilitates promoter activation by nucleosome eviction, and transcription elongation by nucleosome disr...
ORGANISM(S): Saccharomyces cerevisiae 
2014-03-17 | E-GEOD-54340 | biostudies-arrayexpress
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