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GenomicsPost-licensing specification of eukaryotic replication origins by facilitated Mcm2-7 sliding along DNA
Eukaryotic genomes are replicated from many origin sites that are licensed by the loading of inactive double hexamers of the replicative DNA helicase, Mcm2-7. How eukaryotic origin positions are specified remains elusive. Here we show that, contrary to the bacterial paradigm, eukaryotic origins are ...
ORGANISM(S): Saccharomyces cerevisiae 
2016-01-07 | E-GEOD-69065 | biostudies-arrayexpress
TranscriptomicsKc167 Mcm2-7 Localization in HU
Determination of the genome-wide distribution of the Mcm2-7 helicase by chromatin immunoprecipitation in the Drosophila Kc167 cell line at the beginning of S phase. Kc167 cells were arrested at the G1/S transition with hydroxyurea (HU). Goal was to evaluate changes in the genome-wide Mcm2-7 distri...
ORGANISM(S): Drosophila melanogaster 
2014-11-27 | E-GEOD-63680 | biostudies-arrayexpress
TranscriptomicsChromatin IP for Mcm2-7, Rec8, Hop1 and Red1
Mcm2-7 ChIP in pre-meiotic and pre-mitotic cells, axis factor ChIP in wild-type and replication compromised strains in meiosis Multiple studies of meiotic chromosomes were undertaken. To study DNA replication, the locations of replicative helicase (Mcm2-7) were mapped in pre-meiotic and pre-mitotic ...
ORGANISM(S): Saccharomyces cerevisiae 
2012-06-18 | E-GEOD-35658 | biostudies-arrayexpress
TranscriptomicsChIP-chip from Drosophila egg chambers using MCM2-7 antibody
Chromatin immunoprecipitation of the MCM2-7 replicative helicase followed by hybridization to genome-wide tiling microarrays demonstrated that MCMs localize to the follicle cell amplicons at stages of replication initiation Comparison of MCM2-7 ChIP sample compared to input DNA for Drosophila egg ch...
ORGANISM(S): Drosophila melanogaster 
2011-09-06 | E-GEOD-31889 | biostudies-arrayexpress
ProteomicsThe structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer
The controlled assembly of replication forks is critical for genome stability. The Dbf4-dependent Cdc7 kinase (DDK) initiates replisome assembly by phosphorylating the MCM2-7 replicative helicase at the N-terminal tails of Mcm2, Mcm4 and Mcm6. At present, it remains poorly understood how DDK docks o...
ORGANISM(S): Saccharomyces cerevisiae Saccharomyces cerevisiae (Baker's yeast) 
2022-05-31 | PXD031315 | Pride
Cryo-em Ddk Yeast S-phase Dbf4 Molecular dynamics Helicase Origins Cdc7 Helicase activation Kinase Mcm2-7 Dna replication 
ProteomicsConserved phosphorylation mechanism regulates the interaction between the Cdc45-MCM2-7-GINS (CMG) replicative helicase complex and its forked DNA substrate
Mapping of the Chk1 phosphorylation sites in mouse MCM2-7 in vitro with recombinant proteins
ORGANISM(S): Mus musculus (Mouse) 
2025-03-31 | PXD049049 | Pride
Cmg Chk1 Mcm2-7 
UnknownThe ORC/Cdc6/MCM2-7 complex facilitates MCM2-7 dimerization during prereplicative complex formation.
Not available
S-EPMC3936773 | biostudies-literature
GenomicsKc167 Mcm2-7 Localization in HU
Determination of the genome-wide distribution of the Mcm2-7 helicase by chromatin immunoprecipitation in the Drosophila Kc167 cell line at the beginning of S phase. Kc167 cells were arrested at the G1/S transition with hydroxyurea (HU). Goal was to evaluate changes in the genome-wide Mcm2-7 distribu...
ORGANISM(S): Drosophila melanogaster 
2014-11-27 | GSE63680 | GEO
GenomicsMechanisms of MCM2-7 loading and initial DNA melting at near base-pair resolution
Mechanisms of MCM2-7 loading and initial DNA melting at near base-pair resolution
PRJNA1003856 | ENA
GenomicsSpecific origin selection and excess functional MCM2-7 loading in ORC-deficient cells
Specific origin selection and excess functional MCM2-7 loading in ORC-deficient cells
PRJNA1165313 | ENA
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