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TranscriptomicsTranslation profiling of Schizosaccharomyces pombe size separated polysome fractions provides data on average numbers of associated ribosomes for most transcripts
To obtain translational profiles for all mRNAs, polysome preparations are separated according to their size using a sucrose gradient and the mRNAs in each fraction are identified and quantified with DNA microarrays. Starting with exponentially growing cells, we analyzed 12 polysome fractions using D...
ORGANISM(S): Schizosaccharomyces pombe 
2007-03-22 | E-TABM-222 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation to measure RNA polymerase II occupancy in fission yeast
The relative amount of RNA polymerase II (Pol II) associated with a given ORF provides an estimate for transcriptional efficiency. We therefore established a systematic approach to measure Pol II occupancy using chromatin immunoprecipitation followed by analysis on microarrays
ORGANISM(S): Schizosaccharomyces pombe 
2007-03-22 | E-TABM-219 | biostudies-arrayexpress
TranscriptomicsPoly(A) tail length profiling of mRNAs from fission yeast
To obtain global data on polyadenylation of mRNAs, we fractionated the mRNAs according to their poly(A) tail length using a poly-U sepharose column followed by differential elution at five temperatures. Five mRNA fractions with distinct ranges of poly(A) tail length were then hybridized to microarr...
ORGANISM(S): Schizosaccharomyces pombe 
2007-05-16 | E-TABM-217 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of logarithmically growing fission yeast
To estimate mRNA steady-state levels we used RNA extracted from logarithmically growing fisson yeast cells on Affymetrix Yeast 2.0 Genechip arrays. The signal intensities from two independent biological repeats were averaged, resulting in measurements for 4818 out of 4962 nuclear protein-coding gen...
ORGANISM(S): Schizosaccharomyces pombe 
2007-03-22 | E-TABM-220 | biostudies-arrayexpress
OtherDeep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics
Data from ProteomeXchange, PXD ID: PXD000666. File: 20120710_EXQ4_DaHo_SA_labelfree_7D_MN1_3_12_2.mzml. Published as part of Mol Cell Proteomics. 2014 Sep 5 . From the Abstract: {{i}} The fatal neurodegenerative disorders amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA) are the ...
ORGANISM(S): Mus_musculus_viruses, Mouse 
GPM32320003403 | GPMDB
Resource Reanalysis 
ProteomicsDeep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics
The fatal neurodegenerative disorders amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA) are the most common motoneuron disease and genetic cause of infant death, respectively. Various in vitro model systems have been established to investigate motoneuron disease mechanisms - in p...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-02-08 | MSV000084939 | MassIVE
TranscriptomicsComparative microarray analysis of microRNA expression profiles in primary cutaneous malignant melanoma, cutaneous malignant melanoma metastases and benign melanocytic naevi
Perturbations in microRNA (miRNA) expression profiles has been reported for cutaneous malignant melanoma (CMM). With regards to a rapidly growing number of newly discovered miRNA sequences, the availability of up-to-date miRNA expression profiles for primary cutaneous malignant melanoma (PCMM), cuta...
ORGANISM(S): Homo sapiens 
2012-11-02 | E-GEOD-34460 | biostudies-arrayexpress
TranscriptomicsMicroarray analysis of microRNA expression in cutaneous squamous cell carcinoma
MicroRNAs (miRNAs) are a novel class of short RNAs which have shown to be dysregulated in a variety of cancers including squamous cell carcinoma (SCC) of the head&neck. Microarray based miRNA expression profiles of cutaneous SCC (cSCC) however have not been investigated so far. Seven patients with c...
ORGANISM(S): Homo sapiens 
2012-10-08 | E-GEOD-34536 | biostudies-arrayexpress
TranscriptomicsMicroarray analysis of microRNA expression in basal cell carcinoma
Perturbations in the expression profiles of microRNAs (miRNAs) have been reported for a variety of different cancers. Basal cell carcinoma (BCC) of the skin has not been systematically evaluated regarding differentially expressed miRNAs. Patients with BCC (n=7) were included in the study. Punch biop...
ORGANISM(S): Homo sapiens 
2012-04-30 | E-GEOD-34535 | biostudies-arrayexpress
ProteomicsEndogenous THBS1 is C-mannosylated by human induced pluripotent stem cells.
We used a human thrombospondin-1 (THBS1) fragment comprising thrombospondin type 1 repeats (TSRs) 1 to 3, which was recombinantly expressed in HEK 293T cells and digested with AspN to determine chromatographic retention times and to optimise MS parameters. We detected two peptides from TSR2 and TSR3...
ORGANISM(S): Homo Sapiens 
PXD024117 | panorama
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