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TranscriptomicsTranslation profiling of Schizosaccharomyces pombe size separated polysome fractions provides data on average numbers of associated ribosomes for most transcripts
To obtain translational profiles for all mRNAs, polysome preparations are separated according to their size using a sucrose gradient and the mRNAs in each fraction are identified and quantified with DNA microarrays. Starting with exponentially growing cells, we analyzed 12 polysome fractions using D...
ORGANISM(S): Schizosaccharomyces pombe 
2007-03-22 | E-TABM-222 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of logarithmically growing fission yeast
To estimate mRNA steady-state levels we used RNA extracted from logarithmically growing fisson yeast cells on Affymetrix Yeast 2.0 Genechip arrays. The signal intensities from two independent biological repeats were averaged, resulting in measurements for 4818 out of 4962 nuclear protein-coding gen...
ORGANISM(S): Schizosaccharomyces pombe 
2007-03-22 | E-TABM-220 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of fission yeast mRNAs at different times after blocking transcription to estimate mRNA half-lives
We estimated global mRNA half-lives by blocking transcription and measuring mRNA levels at different times after transcriptional shut-off.
ORGANISM(S): Schizosaccharomyces pombe 
2008-02-05 | E-TABM-218 | biostudies-arrayexpress
no omics definedHigh throughput sequenceing of fission yeast to survey the dynamic repertoire of a eukaryotic transcriptome at the single nucleotide resolution
Recent data from several organisms indicate that the transcribed portions of genomes are larger and more complex than expected, and many functional properties of transcripts are not based on coding sequences but on regulatory sequences in untranslated regions or non-coding RNAs. Alternative start a...
ORGANISM(S): Schizosaccharomyces pombe 
2008-12-10 | E-MTAB-5 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation to measure RNA polymerase II occupancy in fission yeast
The relative amount of RNA polymerase II (Pol II) associated with a given ORF provides an estimate for transcriptional efficiency. We therefore established a systematic approach to measure Pol II occupancy using chromatin immunoprecipitation followed by analysis on microarrays
ORGANISM(S): Schizosaccharomyces pombe 
2007-03-22 | E-TABM-219 | biostudies-arrayexpress
TranscriptomicsPoly(A) tail length profiling of mRNAs from fission yeast
To obtain global data on polyadenylation of mRNAs, we fractionated the mRNAs according to their poly(A) tail length using a poly-U sepharose column followed by differential elution at five temperatures. Five mRNA fractions with distinct ranges of poly(A) tail length were then hybridized to microarr...
ORGANISM(S): Schizosaccharomyces pombe 
2007-05-16 | E-TABM-217 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of fission yeast under multiple conditions, including exponential proliferation, meiotic differentiation and environmental stress, and in RNA processing mutants
Recent data from several organisms indicate that the transcribed portions of genomes are larger and more complex than expected, and many functional properties of transcripts are not based on coding sequences but on regulatory sequences in untranslated regions or non-coding RNAs. Alternative start a...
ORGANISM(S): Schizosaccharomyces pombe 
2008-05-18 | E-MTAB-18 | biostudies-arrayexpress
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