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ProteomicsIn-Cell Fast Photochemical Oxidation of Integral Membrane Proteins
We introduce IC-FPOMP (in-cell fast photochemical oxidation of MPs), a method enabling in situ footprinting of IMPs within live cells. IC-FPOMP generates reactive oxygen radicals from several precursors nearby the membrane. We achieve high-throughput and rapid footprinting of IMPs. IC-FPOMP of two h...
ORGANISM(S): Homo sapiens (Human) 
2025-02-14 | PXD058347 | Pride
Membrane proteins Footprinting 
UnknownFast photochemical oxidation of proteins for epitope mapping.
Not available
S-EPMC3193551 | biostudies-literature
ProteomicsStructural aspects of FOXO4 binding to DAF16 investigated with FPOP using both bottom-up and top-down MS.
A combination of covalent labelling techniques and mass spectrometry (MS) is currently a progressive approach for the de-riving insights relating to the mapping of protein surfaces or protein-ligand interactions. In this study, we mapped an interac-tion interface between DNA binding domain (DBD) of ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-14 | PXD027624 | Pride
Bottom-up proteomics Dna Transcription factors Protein Foxo4 Fast photochemical oxidation of proteins Top-dow proteomics 
UnknownModifications generated by fast photochemical oxidation of proteins reflect the native conformations of proteins.
Not available
S-EPMC5980583 | biostudies-literature
UnknownAn improved fast photochemical oxidation of proteins (FPOP) platform for protein therapeutics.
Not available
S-EPMC5993200 | biostudies-literature
UnknownFast Photochemical Oxidation of Proteins Coupled with Mass Spectrometry.
Not available
S-EPMC7497727 | biostudies-literature
UnknownIn Vivo Fast Photochemical Oxidation of Proteins Using Enhanced Multiplexing Proteomics.
Not available
S-EPMC7815197 | biostudies-literature
ProteomicsThe Flash Oxidation (FOX) System: A Novel Laser-free Fast Photochemical Oxidation Protein Footprinting Platform
To validate the FOX flash photolysis system for hydroxyl radical footprinting of proteins experiments, TNF-alpha was oxidized in the presence and absence of a specific monoclonal antibody, adalimumab. The peptides that show decrease in oxidation correlate well with the known epitope for adalimumab.
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD023169 | Pride
Flash photolysis Hrpf Tumor necrosis factor-alpha Mass spectrometry Fpop 
UnknownProtein Footprinting by Carbenes on a Fast Photochemical Oxidation of Proteins (FPOP) Platform.
Not available
S-EPMC4758905 | biostudies-literature
UnknownDosimetry determines the initial OH radical concentration in fast photochemical oxidation of proteins (FPOP).
Not available
S-EPMC5613943 | biostudies-literature
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