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ProteomicsNat10 interacting proteins identified by IP and mass spectrometry (IP-MS) in mESCs
The cells were cotransfected with epitope-tagged expression plasmids or empty vector(EV) . Forty-eight hours after transfection, the cells were harvested with 500 μl of lysis buffer (20 mM Tris-HCl, pH=8.0; 100 mM NaCl; 0.5% NP-40; and 1 mM EDTA) supplemented with protease inhibitor cocktail (#04693...
ORGANISM(S): Mus musculus (Mouse) 
2025-06-23 | PXD062677 | Pride
Ip-ms Nat10 Mescs 
Proteomicsmouse embryonic stem cells heterochromatin-associated protein and Nat10 IP-MS
The sonication-resistant heterochromatin (srHC) fragments of mESCs were isolated by sucrose gradient ultracentrifugation, and the heterochromatin-associated proteins were enriched by Co-IP combined with mass spectrometry (Co-IP/MS) using anti-H3K9me3, IgG was used as a negative control. The Nat10 in...
ORGANISM(S): Mus musculus (Mouse) 
2025-06-23 | PXD056153 | Pride
Nat10 interacted proteins in mescs Heterochromatin-associated proteins in mescs 
ProteomicsLC/MS - Molecular characterization of S
Secreted extracellular vesicles play an important role in pathogen-host interactions. Increased knowledge of schistosome extracellular vesicles could provide insights into schistosome-host interactions and enable the development of novel intervention strategies to inhibit parasitic processes and les...
ORGANISM(S): Schistosoma japonicum (Blood fluke) 
2018-10-26 | PXD003368 | Pride
Lc/ms 
ProteomicsS. japonicum exosome-like vesicles LC-MS/MS
Secreted extracellular vesicles play an important role in pathogen-host interactions. Increased knowledge of schistosome extracellular vesicles could provide insights into schistosome-host interactions and enable the development of novel intervention strategies to inhibit parasitic processes and les...
ORGANISM(S): Schistosoma japonicum (Blood fluke) 
2018-10-27 | PXD003364 | Pride
S. japonicum exosome lc-ms/ms 
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