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ProteomicsAdvanced mass spectrometry workflows for accurate quantification of trace-level host cell proteins in drug products: benefits of FAIMS separation and gas-phase fractionation DIA
In this study, we investigated the benefits of adding high-field asymmetric ion mobility spectrometry (FAIMS) separation prior to data dependent acquisition (DDA) and gas phase fractionation (GPF) prior to data independent acquisition (DIA) LC-MS/MS analysis. Native digestion followed by LC-MS/MS wi...
ORGANISM(S): Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-05-07 | PXD039582 | Pride
Data-independent analysis (dia) Host cell proteins (hcp) Final drug products (dp) 
ProteomicsAdvanced mass spectrometry workflows for accurate quantification of trace-level host cell proteins in drug products: benefits of FAIMS separation and gas-phase fractionation DIA
In this study, we investigated the benefits of adding high-field asymmetric ion mobility spectrometry (FAIMS) separation prior to data dependent acquisition (DDA) and gas phase fractionation (GPF) prior to data independent acquisition (DIA) LC-MS/MS analysis. Native digestion followed by LC-MS/MS wi...
ORGANISM(S): Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-05-07 | PXD039585 | Pride
Faims Data-dependent analysis (dda) Host cell proteins (hcp) Final drug products (dp) 
TranscriptomicsS_aureus_&_triclosan
A triclosan-ciprofloxacin cross-resistant mutant strain of Staphylococcus aureus displays an alteration in the expression of several cell membrane structural and functional genes. Triclosan is an antimicrobial agent found in many consumer products. Several studies have demonstrated that triclosan ...
ORGANISM(S): Staphylococcus aureus 
2010-05-26 | E-GEOD-5799 | biostudies-arrayexpress
ProteomicsA Sensitive, Rapid, Robust and Reproducible Workflow for Host Cell Protein Profiling in Biopharmaceutical Process Development
There is a growing industry and regulatory need to detect host cell protein (HCP) impurities in the production of protein biopharmaceuticals, as certain HCPs can impact product stability, safety, and efficacy, even at low levels.1-6 In some cases, regulatory agencies require the identification as we...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-02 | PXD018427 | Pride
Monoclonal antibody (mab) Drug product (dp) Evosepone Drug substance (ds) Host cell proteins (hcps) Lc-ms/ms 
TranscriptomicsmiRNA expression of lung cancer cells A549 and cisplatin (DDP) resistant lung cancer cells A549/DDP
To investigate the difference of miRNA expression between lung cancer cell A549 and its DDP-resistant cell strain A549/DDP, we have employed miRNA microarray expression to discover the difference expression of miRNAs of A549 cells and A549/DDP. We conducted RT-qPCR to examine the expression levels o...
ORGANISM(S): Homo sapiens 
2016-08-16 | E-GEOD-85603 | biostudies-arrayexpress
ProteomicsProteomic characterization of Opisthorchis viverrini excretory/secretory products
O. viverrini infection is recognised by the WHO as a group 1 biological carcinogen because of its extremely strong link with cholangiocarcinoma (CCA), cancer of the bile ducts. The incidence of CCA associated with liver fluke infection, calculated as an age‐standardized rate, varies by geographical ...
ORGANISM(S): Opisthorchis 
2024-09-20 | PXD056031 | Pride
Proteomics Excretory/secretory products Opisthochis viverrini Secretome 
MetabolomicsThe Metabolic Fate of Oxaliplatin in the Biological Milieu Investigated During In Vivo Lung Perfusion Using a Unique Miniaturized Sampling Approach Based on Solid-Phase Microextraction Coupled with Liquid Chromatography-Mass Spectrometry

Adjuvant chemotherapy after pulmonary metastasectomy for colorectal cancer may reduce recurrence and improve survival rates; however, the benefits of this treatment are limited by the significant side effects that accompany it. The development of a novel in vivo lung perf...

2022-08-02 | MTBLS4982 | MetaboLights
TranscriptomicsRNA-seq of WT 23hpf zebra fish embryo heads treated with DMSO (control), Dasatinib 150uM, IWR1 25uM, YODA1 10uM, Rockout 100uM or CaliculinA 0.1uM.
The folding of the optic cup during embryonic development is a highly mechanic process. In our laboratory we want to demonstrate how the Retinal Pigmented Epithelium (RPE) cells are essential for the correct morphogenesis of the eye. Our experimental workflow has shown that the hippo and B-catenin p...
ORGANISM(S): Danio rerio 
2026-07-03 | E-MTAB-17258 | biostudies-arrayexpress
TranscriptomicsLeishmania donovani amastigotes: Nelfinavir (NFV)-sensitive (control) vs Nelfinavir-resistant [RNA expression profiling]
Drug resistance is a major public health challenge in Leishmaniasis chemotherapy, particularly in the case of emerging Leishmania/HIV-1 co-infections. Recently, we have delineated the mechanism of cell death induced by the HIV-1 protease inhibitor, Nelfinavir, in the Leishmania parasite. In order to...
ORGANISM(S): Leishmania donovani 
2013-06-27 | E-GEOD-42196 | biostudies-arrayexpress
TranscriptomicsLeishmania donovani amastigotes: Nelfinavir (NFV)-sensitive (control) vs Nelfinavir-resistant [Comparative Genomic Hybridization (DNA) profiling]
Drug resistance is a major public health challenge in Leishmaniasis chemotherapy, particularly in the case of emerging Leishmania/HIV-1 co-infections. Recently, we have delineated the mechanism of cell death induced by the HIV-1 protease inhibitor, Nelfinavir, in the Leishmania parasite. In order to...
ORGANISM(S): Leishmania donovani 
2013-06-27 | E-GEOD-42195 | biostudies-arrayexpress
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