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ProteomicsGetting more out of FLAG-Tag co-immunoprecipitation mass spectrometry experiments using FAIMS.
Co-immunoprecipitation of proteins coupled to mass spectrometry is critical for the understanding of protein interaction networks. In instances where a suitable antibody is not available, it is common to graft synthetic tags onto target protein sequences and allowing the use of commercially availabl...
ORGANISM(S): Homo sapiens (Human) 
2023-02-21 | PXD028965 | Pride
Faims Co-ip mass spectrometry Flag tag Proteomics Ion mobility 
GenomicsNse4-FLAG CHIP-seq analysis in gcn5, ubp8, ada2 deletion background in S.pombe, control wt 501 - no tag
to find functional relevance between SMC5/6 complex and SAGA histone acetyltransferase complex in Schizosaccharomyces pombe by chromatin immunoprecipitation of Nse4-FLAG tagged protein from gcn5, ubp8 and ada2 deletion strains and 2 control strains (WT - Nse4-FLAG, 501 - no tag).
ORGANISM(S): Schizosaccharomyces pombe 
2023-02-20 | E-MTAB-12401 | biostudies-arrayexpress
GenomicsFLAG-tagged PITX2 CUT&Tag
FLAG-tagged PITX2 CUT&Tag
PRJNA1333295 | ENA
UnknownTaking down the FLAG! How insect cell expression challenges an established tag-system.
Not available
S-EPMC3368911 | biostudies-literature
UnknownSulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition.
Not available
S-EPMC8099120 | biostudies-literature
ProteomicsLC-MSMS with isobaric tagging on Flag-coimmunoprecipitation eluates from Flag-Pgrmc1 and Y113F Flag-Pgrmc1 expressing Pgrmc1 KO mouse liver membranes
Progesterone receptor membrane component 1 (PGRMC1) is a heme binding protein implicated in a wide range of cellular functions. Our previous studies showed that PGRMC1 binds to cytochromes P450 in yeast and mammalian cells and promotes activity of these enzymes. Recently, the paralog PGRMC2 was show...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-15 | PXD028284 | Pride
Lc-msms Mouse Tmt Co-immunoprecipitation Y113f flag-pgrmc1 Bound Membrane Pgrmc1 Flag-pgrmc1 Liver Eluate Isobaric tag 
UnknownHis-FLAG Tag as a Fusion Partner of Glycosylated Human Interferon-Gamma and Its Mutant: Gain or Loss?
Not available
S-EPMC5480026 | biostudies-literature
GenomicsNse4-FLAG CHIP-seq analysis in gcn5, ubp8, ada2 deletion background in S.pombe, control wt 501 - no tag
Nse4-FLAG CHIP-seq analysis in gcn5, ubp8, ada2 deletion background in S.pombe, control wt 501 - no tag
PRJEB57799 | ENA
UnknownStructural Analysis of the Effect of a Dual-FLAG Tag on Transthyretin.
Not available
S-EPMC7171973 | biostudies-literature
UnknownGeneration of Flag/DYKDDDDK Epitope Tag Knock-In Mice Using i-GONAD Enables Detection of Endogenous CaMKIIα and β Proteins.
Not available
S-EPMC9569722 | biostudies-literature
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