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BACKGROUND: Stool metabolites provide essential insights into the function of the gut microbiome. The current gold standard for storage of stool samples for metabolomics is flash-freezing at - 80 °C which can be inconvenient and expensive. Ambient temperature storage of stool is ...

2021-01-27 | MTBLS201 | MetaboLights

We present expeditious querying, reliable and platform-independent lipid annotation software LipidIN, a comprehensive lipidome atlas with tandem mass spectrometry information, relative retention time and reverse mass spectrometry information. Five-levels spectral fragmentations trees were formula...

2025-03-21 | MTBLS10170 | MetaboLights
We have used a combination of RNAi based functional and molecular genomic approaches to investigate in detail two proteins required for the survival of CRC cells. Set1 has 22 samples, 10 siNeg controls, 6 siRNA transfections for FLASH and NUP62 each. Set2 has 67 samples, siNeg controls at timepoint:...
ORGANISM(S): Homo sapiens 
To validate the FOX flash photolysis system for hydroxyl radical footprinting of proteins experiments, TNF-alpha was oxidized in the presence and absence of a specific monoclonal antibody, adalimumab. The peptides that show decrease in oxidation correlate well with the known epitope for adalimumab.
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD023169 | Pride
Protein post-translational modifications (PTMs) are key modulators of protein structure and function that often change in a dynamic fashion in response to cellular stimuli. Dynamic post-translational modifications are very challenging to structurally characterize using modern techniques, including c...
ORGANISM(S): Gallus gallus (Chicken) 
2023-01-16 | PXD037050 | Pride
Single cell sequencing with FLASH-seq of K562 and mESC cells
Hypoxic tumors are sensitive to FLASH radiotherapy
We utilized FLASH-seq, a full-length bulk RNA sequencing method, to sequence single spheroids in a 96-well format by manually sorting human and chimpanzee spheroids budding at day 3 to day 6 of midgut/hindgut patterning. We would like to use this data to assess the early stages of differentiation an...
ORGANISM(S): Homo sapiens 
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