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MetabolomicsAn ambient-temperature storage and stabilization device performs comparably to flash-frozen collection for stool metabolomics in infants

BACKGROUND: Stool metabolites provide essential insights into the function of the gut microbiome. The current gold standard for storage of stool samples for metabolomics is flash-freezing at - 80 °C which can be inconvenient and expensive. Ambient temperature storage of stool is ...

2021-01-27 | MTBLS201 | MetaboLights
UnknownNonrandom RNAseq gene expression associated with RNAlater and flash freezing storage methods.
Not available
S-EPMC6393184 | biostudies-literature
UnknownTime course analysis of RNA quality in placenta preserved by RNAlater or flash freezing.
Not available
S-EPMC5397349 | biostudies-literature
TranscriptomicsRibosome profiling of astrovirus-infected cells
Ribosome profiling (RiboSeq) is a high-throughput sequencing technique for globally mapping the positions of translating ribosomes on the transcriptome. We infected Caco2 cells with human astrovirus 1 (HAstV1). Cells were harvested at 12 hpi and either flash frozen with no pre-treatment (NT), or pre...
ORGANISM(S): Homo sapiens 
2020-07-04 | E-MTAB-8045 | biostudies-arrayexpress
TranscriptomicsGene expression differences under freezing and ice encasement stress in between temporal populations
The experiment was aimed at studying gene expression differences between three temporal populations of two timothy cultivars; Engmo (Early=1988, Mid=2003 & Current=2020) and Noreng (Early=1998, Mid=2010 & Current=2020), under freezing stress and Ice encasement stress. The idea is when the temporally...
ORGANISM(S): Phleum pratense 
2025-09-01 | E-MTAB-14732 | biostudies-arrayexpress
MetabolomicsLactobacillus reuteri bile acid metabolism in vitro

Upon reaching an OD600 of 0.3, L.

reuteri cultures were treated with 100 µM TCA, HCA, or

TCDCA. For BSHs inhibition assays, additional wells were treated with 100 µM

HCA + GR7. Both negative control and MRS medium control groups were included.

Cultures were terminated at 7...

2025-07-04 | MTBLS12641 | MetaboLights
GenomicsCell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells
We describe a protocol for freezing neuronal cells that is compatible with ATAC-Seq, producing results that compare well with those generated from fresh cells. We developed our protocol on a disease-relevant cell type, namely motor neurons differentiated from induced pluripotent stem cells from a pa...
ORGANISM(S): Homo sapiens 
2016-05-05 | GSE78036 | GEO
TranscriptomicsStudy for evaluating the effect of cold ischemic time and RNA stabilization method on RNA integrity and gene expression measurements
Time series of eleven breast cancer samples subjected to different cold ischemic stress of up to 3 hr post tumor excision. A different 2x2 factorial within this study evaluated the effect of stabilization method (RNAlater vs snap freezing) and stablization delay (0 and 40 min) at room temperature. T...
ORGANISM(S): Homo sapiens 
2011-11-02 | E-GEOD-25011 | biostudies-arrayexpress
UnknownAn ambient-temperature storage and stabilization device performs comparably to flash-frozen collection for stool metabolomics in infants.
Not available
S-EPMC7901118 | biostudies-literature
UnknownCognitive and behavioral effects of whole brain conventional or high dose rate (FLASH) proton irradiation in a neonatal Sprague Dawley rat model.
Not available
S-EPMC9481014 | biostudies-literature
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