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ProteomicsFAM98C KO validation in HEK Flp-In T-REx 293 cells.
Validation of a FAM98C KO in HEK Flp-In T-REx 293 cells by quantifying the occurrence of FAM98C-specific peptides in whole cell lysates with LC-MSMS. WT HEK Flp-In T-REx 293 cells were used as control.
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD076694 | Pride
TranscriptomicsRNA-Seq of WT Flp-In T-REx 293 and UPF3B knockout cell lines with additional siRNA-mediated control or UPF3A knockdowns
UPF3A and UPF3B are paralogous genes in human cells that are involved in the nonsense-mediated decay (NMD) pathway. NMD is a cellular quality control mechanism that monitors mRNAs during translation. Aberrant translation due to features such as the presence of a premature stop codon downstream on an...
ORGANISM(S): Homo sapiens 
2022-04-25 | E-MTAB-10711 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of WT Flp-In T-REx 293, UPF3B knockout and UPF3A-UPF3B double knockout cell lines with additional siRNA-mediated control or UPF3B knockdowns
UPF3A and UPF3B are paralogous genes in human cells that are involved in the nonsense-mediated decay (NMD) pathway. NMD is a cellular quality control mechanism that monitors mRNAs during translation. Aberrant translation due to features such as the presence of a premature stop codon downstream on an...
ORGANISM(S): Homo sapiens 
2022-04-25 | E-MTAB-10716 | biostudies-arrayexpress
ProteomicsAnti-FLAG co-immunoprecipitation of N- or C-terminally FLAG-HA-tagged FAM98A, FAM98B and FAM98C, from HEK Flp-In T-REx 293 cells.
Determination of the interactome of different human FLAG-HA-tagged FAM98 paralogs (FAM98A, FAM98B and FAM98C) by co-immunoprecipitation/MS. Two expression constructs were used per paralog: one with an N-terminal and one with a C-terminal FLAG-HA-tag. Expression constructs were stably integrated into...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD076703 | Pride
TranscriptomicsRNA-Seq of control and RNPS1 knockdown with or without RNPS1 wildtype or RNPS1-RRM domain rescue in Flp-In T-REx 293 cells
RNPS1 is a splicing regulatory protein and a component of the ASAP/PSAP complex, which is associated with the exon junction complex and modulates alternative splicing. It was previously postulated that the isolated RRM domain of RNPS1 in complex with ASAP/PSAP is able to regulate certain alternative...
ORGANISM(S): Homo sapiens 
2022-05-06 | E-MTAB-10770 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of WT Flp-In T-REx 293, UPF3A knockout and UPF3A overexpression cell lines in part with additional siRNA-mediated control or UPF3B knockdowns
UPF3A and UPF3B are paralogous genes in human cells that are involved in the nonsense-mediated decay (NMD) pathway. NMD is a cellular quality control mechanism that monitors mRNAs during translation. Aberrant translation due to features such as the presence of a premature stop codon downstream on an...
ORGANISM(S): Homo sapiens 
2022-08-31 | E-MTAB-10718 | biostudies-arrayexpress
TranscriptomicsMonosome and disome profiling of Flag-STAU1 Flp-In 293 T-REx cells
Monosome and disome profiling was performed on Flag-STAU1 Flp-In 293 T-REx to study the causes of ribosomal collisions, and whether this may be modulated by the presence/absence of Staufen-1. Cells were treated with either an siRNA targeting STAU1 transcript (4x samples) or a control siRNA (2x sam...
ORGANISM(S): Homo sapiens 
2023-04-30 | E-MTAB-9669 | biostudies-arrayexpress
ProteomicsA family of unconventional deubiquitinases with modular chain specificity determinants
Interactors of human ZUFSP in HEK-293T cells. Flp-in T-REx 293 cells with stable 3xFLAG-ZUFSP integration were assayed for ZUFSP interactors under three different conditions: i) Cells expressing wild-type ZUFSP, ii) Cells expressing catalytically inactive ZUFSP, iii) Cells expressing wild-type ZUFSP...
ORGANISM(S): Homo sapiens (Human) 
2018-04-06 | PXD008731 | Pride
Human Hek-293t cells Zufsp 
ProteomicsMitochondrial translocase blocking ATP5MG-tFT fusion protein interactome in human embryonic kidney (HEK) 293 cells
The experiment uses HEK-293 Flp-In T-Rex cells to express fusion protein that combines ATP synthase subunit ATP5MG with a tandem fluorescent protein timer (tFT). The tFT tag combines mCherry and super folder GFP (sfGFP) fluorescent proteins. An exceptionally stable structure of sfGFP interferes with...
ORGANISM(S): Homo sapiens (Human) 
2024-03-08 | PXD038583 | Pride
Translocase of the outer mitochondrial membrane Protein import Quality control Mitochondria Translocation 
TranscriptomicsExpression data of inducible stable cell lines overexpressing Ero1alpha WT and Ero1alpha C104A/C131A
The overall cellular response to oxidative stress generated by Ero1M-NM-1 in the lumen of the mammalian endoplasmic reticulum (ER) is poorly characterized. Here, we investigate the effects of overexpressing a hyperactive mutant (C104A/C131A) of Ero1M-NM-1. Using microarray analysis, we demonstrate t...
ORGANISM(S): Homo sapiens 
2012-09-07 | E-GEOD-40601 | biostudies-arrayexpress
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