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ProteomicsEffects of hypoxia on antigen presentation and T cell-based immune recognition of HPV16-transformed cells
Attempts to develop a therapeutic vaccine against human papillomavirus (HPV)-induced malignancies have not been clinically successful to date. One reason may be the hypoxic microenvironment present in most tumors, including cervical cancer. Hypoxia dysregulates the levels of human leukocyte antigen ...
ORGANISM(S): Homo sapiens (Human) 
2022-11-16 | PXD032217 | Pride
Antigen presentation machinery (apm) Cytotoxic cd8+ t cells Hpv16 
ProteomicsmRNA interactome in mouse macrophages
Proteins interacting with mRNA in mouse macrophages were identified by crosslinking and oligodT capture. Differential interaction was investigated in macrophages before and after treatment with LPS.
ORGANISM(S): Mus musculus (Mouse) 
2016-06-13 | PXD002890 | Pride
Macrophage Protein interaction Mrna 
ProteomicsHL-1 mRNA interactome - The Cardiomyocyte RNA-Binding Proteome: Links to Intermediary Metabolism and Heart Disease
RNAsfunction as compositionally diverse ribonucleoprotein (RNP) comlplexes, formed through interactions with RNA-binding proteins (RBPs). We explored the murine cardiomyocyte RBP repertoire.
ORGANISM(S): Mus musculus (Mouse) 
2016-08-17 | PXD002541 | Pride
Mrna interactome 
ProteomicsRBDmap - The Cardiomyocyte RNA-Binding Proteome: Links to Intermediary Metabolism and Heart Disease
RBDmap protein regions contacting RNA were crosslinked with UV and captured with oligodT beads
ORGANISM(S): Mus musculus (Mouse) 
2016-08-17 | PXD002543 | Pride
Rna-binding proteins 
ProteomicsHuman oocyte proteome and secretome
The proteome and secretome of human oocytes was studied by shotgun LCMSMS, starting from pools of 100 cells. Exploiting SP3, a novel technology for proteomic sample preparation using magnetic beads, we scaled down proteome analysis to single cells. Despite the low protein content of only ~100ng per ...
ORGANISM(S): Homo sapiens (Human) 
2016-06-01 | PXD004142 | Pride
Human oocytes 
ProteomicsHuman oocytes - Identification of Maturation-Specific Proteins by Single-Cell Proteomics of Human Oocytes
comparison of mature and immature human oocytes, the amount differed from 1 to 100 oocytes per experiment.
ORGANISM(S): Homo sapiens (Human) 
2018-08-29 | PXD003121 | Pride
Single occoyte analysis Human oocytes Lcms/ms 
ProteomicsRNA-binding protein repertoire of embryonic stem cells
RNA-binding proteins (RBPs) have essential roles in RNA-mediated gene regulation, and yet annotation of RBPs is limited mainly to those with known RNA-binding domains. To systematically identify the RBPs of embryonic stem cells (ESCs), we here employ interactome capture, which combines UV cross-link...
ORGANISM(S): Mus musculus (Mouse) 
2018-08-29 | PXD008914 | Pride
Mouse Lc-ms Rna-binding Mecs 
ProteomicsComparative secretome analysis of primary murine white and brown adipocytes
The adipose organ, including white and brown adipose tissues, is an important player in systemic energy homeostasis, storing excess energy in form of lipids while releasing energy upon various energy demands. Recent studies have demonstrated that white and brown adipocytes also function as endocrine...
ORGANISM(S): Mus musculus (Mouse) 
2018-09-04 | PXD009280 | Pride
Brown adipocytes Adrenergic stimulation White adipocytes Secretome Murine svf cells 
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