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GenomicsFormaldehyde-crosslinking and immunoprecipitation (FA-CLIP) sequencing to identify ECT2 targets in Arabidopsis
Formaldehyde-crosslinking and immunoprecipitation (FA-CLIP) sequencing to identify ECT2 targets in Arabidopsis
PRJNA422491 | ENA
ProteomicsFormaldehyde-crosslinking enables comprehensive profiling of in vivo RNA-interactome in UV-impermeable samples
Development of UV-crosslinking based RNA-interactome capture protocol have expanded the repertoire of RBPs. Apparent limitations of UV-crosslinking, however, have made the RBP profiling to be incomplete and inapplicable to many of the biological samples in physiological contexts. Here we developed f...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2020-11-25 | PXD010046 | Pride
Rbp formaldehyde 
ProteomicsGlobal Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling
We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility du...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-08-02 | MSV000080014 | MassIVE
ProteomicsConventional cell fixation improves performance of in situ crosslinking mass spectrometry while preserving cellular ultrastructure
We developed an approach to in situ XL-MS which utilizes pre-stabilization with formaldehyde prior to crosslinker introduction. Applications of this method can boost crosslinker signal and allows for membrane-impermeable reagents to be used for studying protein-interaction landscapes in intact human...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2024-04-12 | PXD051075 | Pride
In situ xl-ms Formaldehyde fixaiton Crosslinking mass spectrometry 
TranscriptomicsTranscriptome-wide mapping of human Staufen1 binding sites
Purpose: We performed RNA-Immunoprecipitation in Tandem (RIPiT) experiments against human Staufen1 (Stau1) to identify its precise RNA binding sites in a transcriptome-wide manner. To monitor the consequences of Stau1 binding in terms of target mRNA levels and ribosome occupancy, we modified the lev...
ORGANISM(S): Homo sapiens 
2013-11-25 | E-GEOD-52447 | biostudies-arrayexpress
ProteomicsGlobal Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling
We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility du...
ORGANISM(S): Homo sapiens (Human) 
2016-04-27 | PXD003754 | Pride
Proteomics  protein complexes  protein crosslinking  mass spectrometry Membrane protein  interactome analysis 
UnknownGenetically encoded formaldehyde sensors inspired by a protein intra-helical crosslinking reaction.
Not available
S-EPMC7835342 | biostudies-literature
ProteomicsHuman Angiotensin-Converting Enzyme 2-Specific Benzothiazole Allosteric Inhibitor against Pan-Sarbecoviruses
Emerging SARS-CoV-2 variants of concern (VOCs) have demonstrated exceptional transmissibility and a strong dependence on the cellular receptor human angiotensin-converting enzyme 2 (hACE2). In this study, we present MB-32, a benzothiazole-based small molecule allosteric inhibitor of hACE2, w...
ORGANISM(S): Homo sapiens (Human) Sarbecovirus 
2026-05-01 | PXD075140 | Pride
Human Mb-32 Entry inhibtor Angiotensin-converting enzyme 2 Covid19 Allosteric inhibitor Sarbecovirus Benzothiazole Formaldehyde crosslinking 
ProteomicsClick-linking: a cell-compatible protein crosslinking method based on click chemistry
We developed a novel approach to in situ crosslinking mass spectrometry (XL-MS) where the reaction is split into two sequential and orthogonal coupling events. The method involves pre-stabilization of the proteome using a fixation protocol, followed by a two-step process that begins with extensive l...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2025-01-28 | PXD059118 | Pride
In situ Crosslinking Proteome-wide Protein-protein interactions Interactome 
ProteomicsGlobal Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling
We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility du...
ORGANISM(S): Homo sapiens (Human) 
2022-03-01 | PXD002929 | Pride
Crosslinking Protein-protein interactions 
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