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Unluckily, FFPE archival methods lead to partial RNA degradation, limiting the amount of derivable information. This study aims to evaluate if the DASL gene expression assay, designed to generate reproducible data from degraded RNAs, is a reliable method to apply on RNA from FFPE tissues. In order t...
ORGANISM(S): Homo sapiens 
Future improvements in the field of breast cancer prevention, diagnosis and treatment require deeper knowledge and better understanding of the molecular processes and signaling pathways that lead to the transformation of normal cells to cancer ones and the disease progression. Quantitative proteome ...
ORGANISM(S): Homo sapiens (Human) 
2026-03-16 | PXD008408 | Pride
We used quantitative RNA expression profiling on the Affymetrix U133 human expression array to validate quantitative expression results obtained with the tissue preservative RNALater against snap frozen and fresh tissues as a means of routine tissue collection and temporary storage. By using split ...
ORGANISM(S): Homo sapiens 
Fresh versus slow-frozen ATACseq samples for salmon tissues
Tissue biopsies are most commonly archived in a paraffin block following tissue fixation with formaldehyde (FFPE) or as fresh frozen tissue (FFT). While both methods preserve biological samples, little is known about how they affect the quantifiable proteome. We performed a ‘bottom-up’ proteomic ana...
ORGANISM(S): Homo sapiens (Human) 
2024-02-12 | PXD032108 | Pride
Whole genome bisulfite sequencing and Infinium Mouse Methylation BeadChip using fresh and fresh-frozen mouse epidermis (WGBS)
Glioblastoma multiforme (GBM) is the most aggressive brain tumor with poor prognosis to most patients. Immunotherapy of GBM is a potentially beneficial treatment option, whose optimal implementation may depend on familiarity with tumor specific antigens, presented as HLA peptides by the GBM cells. F...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
Single Cell RNA Sequencing of Fresh and Frozen Human Atheroma Tissue
The Ocular Surface Microbiome: A Comparison of Fresh and Frozen Samples
The Ocular Surface Microbiome: A Comparison of Fresh and Frozen Samples
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