RBM39 is a RNA binding protein, we aim to investigate its role in multiple myeloma. We performed mass spectrometry of RBM39 interacted protein obtained by IP.
We applied SelectID coupled with the ultra-sensitive micro-DIA approach to enrich the associated proteins of Chromosome 9 satellite region and methylated LINE-1 elements.
Identification and quantification of biotinylated proteins enriched by the Sp-system at Chromosome 9 Satellite regions using TMT-labeled quantitative mass spectrometry
To validate whether the dCas9-TurboID system is applicable for proximity labeling at specific genomic regions, label-free mass spectrometry was employed to validate the satellite relative proteins that were enriched in the group of stable Hela cell line expressing sgAS.
