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ProteomicsIdentification of HLA class I immunopeptide from human colon tissue (Tumor)
HLA Class I immunopeptides were affinity purified by W6/32 antibody and analyzed by Orbitrap Fusion Lumos with FAIMS. Personalized database which includes patient-specific somatic mutations obtained from whole exome sequencing (WES) data was used for database search. Identification results were filt...
ORGANISM(S): Homo Sapiens (human) 
2022-08-20 | PXD023805 | JPOST Repository
ProteomicsGlobal identification of HCT116 immunopeptides by FAIMS-assisted immunopeptidomics
HLA Class I immunopeptides were affinity purified by W6/32 antibody and analyzed by Orbitrap Fusion Lumos with FAIMS. Customized database which includes somatic mutations of HCT116 obtained from COSMIC database was used for database search. Identification results were filtered at 1% FDR thresholds b...
ORGANISM(S): Homo Sapiens (human) 
2022-08-20 | PXD023773 | JPOST Repository
ProteomicsComparison of efficacy in immunopeptide identification from HCT116 by "without" FAIMS
HLA class I immunopeptides were affinity purified by W6/32 antibody and analyzed by Orbitrap Fusion Lumos without FAIMS. Customized database which includes somatic mutations of HCT116 obtained from COSMIC database was used for database search. Identification results were filtered at 1% FDR threshol...
ORGANISM(S): Homo Sapiens (human) 
2022-08-20 | PXD023770 | JPOST Repository
ProteomicsComparison of efficacy in immunfopeptide identification from HCT116 by "with" FAIMS
HLA class I immunopeptides were affinity purified by W6/32 antibody and analyzed by Orbitrap Fusion Lumos with FAIMS. Customized database which includes somatic mutations of HCT116 obtained from COSMIC database was used for database search. Identification results were filtered at 1% FDR threshold by...
ORGANISM(S): Homo Sapiens (human) 
2022-08-20 | PXD023771 | JPOST Repository
ProteomicsIdentification of HLA class I immunopeptide from human colon tissue (Normal)
HLA Class I immunopeptides were affinity purified by W6/32 antibody and analyzed by Orbitrap Fusion Lumos with FAIMS. Personalized database which includes patient-specific somatic mutations obtained from whole exome sequencing (WES) data was used for database search. Identification results were filt...
ORGANISM(S): Homo Sapiens (human) 
2022-08-20 | PXD023804 | JPOST Repository
ProteomicsTissue-exudative extracellular vesicles of colorectal cancer
Freshly resected colorectal cancer tissues and adjacent normal tissues of 17 individuals were incubated in serum-free medium. Extracellular vesicles were isolated from the medium with ultracentrifugation method.
ORGANISM(S): Homo Sapiens (human) 
2021-03-06 | PXD020439 | JPOST Repository
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