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TranscriptomicsZscan10 is dispensable for maintenance of pluripotency in mouse embryonic stem cells
Zinc finger and SCAN domain-containing 10 (Zscan10, also known as Zfp206) encodes a transcription factor that has been reported to be involved in the maintenance of pluripotency in mouse embryonic stem (ES) cells. Here we generated inducible knockout ES cells for Zscan10 using the Cre-loxP system an...
ORGANISM(S): Mus musculus 
2015-12-20 | E-GEOD-76143 | biostudies-arrayexpress
ProteomicsMudPIT analyses of the proteins co-purified with the Interactor of Little Elongation Complex ELL subunit 1 (FLAG-ICE1), WT MED26, and D2G8 NTD-mutant MED26, affinity-purified from HEK293T cell lines
Protein Complexes Purification: We generated 293FRT cell lines that stably express FLAG-tagged ICE1 (Interactor of Little Elongation Complex ELL subunit 1; KIAA0947). We extracted nuclear proteins in the presence of Benzonase to digest nucleic acids, including both DNA and RNA, and purified the lit...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-02-20 | MSV000083465 | MassIVE
UnknownDirect derivation of human alveolospheres for SARS-CoV-2 infection modeling and drug screening.
Not available
S-EPMC8133488 | biostudies-literature
GenomicsGenome-wide localization of NF-E2 p45 in mouse primary megakaryocytes.
ChIP-seq assay was performed with anti-p45 antibody using in vitro cultured primary megakaryocytes to identify direct p45 target genes in megakaryocytes. This analysis revealed genes that regulate platelet function, which were not known previously to be p45-regulated. ChIP was performed using a day...
ORGANISM(S): Mus musculus 
2013-11-01 | E-GEOD-42249 | biostudies-arrayexpress
ProteomicsProteome analysis of mouse liver and skeletal muscle
The liver and skeletal muscle were collected from 10-week-old wild-type and ob/ob mice fasted for 16 hours. Tissues were frozen immediately and lysed with 0.5 mL of a solution containing 50 mM Tris-HCl pH8.8, 2%SDS, and 7 M urea, and then subjected to ultrasonic treatment with a Bioruptor (Digaenode...
ORGANISM(S): Mus Musculus (mouse) 
2021-03-03 | PXD020656 | JPOST Repository
ProteomicsTissue-exudative extracellular vesicles of colorectal cancer
Freshly resected colorectal cancer tissues and adjacent normal tissues of 17 individuals were incubated in serum-free medium. Extracellular vesicles were isolated from the medium with ultracentrifugation method.
ORGANISM(S): Homo Sapiens (human) 
2021-03-06 | PXD020439 | JPOST Repository
ProteomicsIdentification of HLA class I immunopeptide from human colon tissue (Tumor)
HLA Class I immunopeptides were affinity purified by W6/32 antibody and analyzed by Orbitrap Fusion Lumos with FAIMS. Personalized database which includes patient-specific somatic mutations obtained from whole exome sequencing (WES) data was used for database search. Identification results were filt...
ORGANISM(S): Homo Sapiens (human) 
2022-08-20 | PXD023805 | JPOST Repository
ProteomicsGlobal identification of HCT116 immunopeptides by FAIMS-assisted immunopeptidomics
HLA Class I immunopeptides were affinity purified by W6/32 antibody and analyzed by Orbitrap Fusion Lumos with FAIMS. Customized database which includes somatic mutations of HCT116 obtained from COSMIC database was used for database search. Identification results were filtered at 1% FDR thresholds b...
ORGANISM(S): Homo Sapiens (human) 
2022-08-20 | PXD023773 | JPOST Repository
ProteomicsComparison of efficacy in immunopeptide identification from HCT116 by "without" FAIMS
HLA class I immunopeptides were affinity purified by W6/32 antibody and analyzed by Orbitrap Fusion Lumos without FAIMS. Customized database which includes somatic mutations of HCT116 obtained from COSMIC database was used for database search. Identification results were filtered at 1% FDR threshol...
ORGANISM(S): Homo Sapiens (human) 
2022-08-20 | PXD023770 | JPOST Repository
ProteomicsComparison of efficacy in immunfopeptide identification from HCT116 by "with" FAIMS
HLA class I immunopeptides were affinity purified by W6/32 antibody and analyzed by Orbitrap Fusion Lumos with FAIMS. Customized database which includes somatic mutations of HCT116 obtained from COSMIC database was used for database search. Identification results were filtered at 1% FDR threshold by...
ORGANISM(S): Homo Sapiens (human) 
2022-08-20 | PXD023771 | JPOST Repository
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