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Genome-wide scanning of gene expression by microarray techniques was successfully performed on RNA extracted from a sterilized soil inoculated with Pseudomonas putida KT2440/pSL1, which contains a chloroaromatic degrading plasmid, in the presence or absence of 3-chlorobenzoic acid (3CB). The genes s...
ORGANISM(S): Pseudomonas putida KT2440 
Genome-wide microarray analysis was performed using RNA extracted from soil cultures of Streptomyces coelicolor A3(2) in the presence or absence of chitin. The vast majority of genes in chitin and amino sugar metabolism, as well as many other genes for carbon and energy, nitrogen and sulfur metaboli...
ORGANISM(S): Streptomyces coelicolor A3(2) 
Transcriptomic analysis of the cells grown on biphenyl in the sterilized soil and those grown on biphenyl to mid-exponential phase in a liquid medium 4x dual channel slides.
ORGANISM(S): Rhodococcus jostii RHA1 
Transcroptomic analysis of the cells grown on pyruvate in the sterilized soil and those grown on pyruvate to mid-exponenyial phase in a liquid medium 4x dual channel slides.
ORGANISM(S): Rhodococcus jostii RHA1 
Acetylcholine (ACh) has been considered a neurotransmitter residing in central, parasympathetic and neuromuscular synapses of mammals. Here, experiments using crypt-villus organoids that lack nerve and immune cells in culture led us to suggest that endogenous ACh is synthesized in the intestinal epi...
ORGANISM(S): Mus musculus 
OfMasc-GFP derivatives or GFP were transiently expressed in OfTC cells. At 2 days post transfection, OfTC cells were treated with 100 nM Bortezomib for 1 day. The OfT1C cells were then fixed with 0.1% formaldehyde, and the fixation was quenched with 300 mM glycine. The cells were lysed on ice in 1 m...
ORGANISM(S): Cellular Organisms 
OfMasc-GFP derivatives or GFP were transiently expressed in OfT1C/tet cells (3.2e6 cells) seeded in 10 cm-diameter culture dishes. At two days post transfection, the cells were fixed, collected, and lysed on ice for 10 min in 1 mL of RIPA buffer (20 mM HEPES-KOH, pH 7.5, 1 mM EGTA, 1 mM MgCl2, 150 m...
ORGANISM(S): Cellular Organisms 
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