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TranscriptomicsRetrograde bilin signaling enables Chlamydomonas greening and phototrophic survival
Here we show that the phytochrome-less chlorophyte Chlamydomonas reinhardtii retains a functional pathway to synthesize the linear tetrapyrrole (bilin) precursor of the phytochrome chromophore. Reverse genetic, metabolic inactivation and bilin rescue experiments establish that this pathway is needed...
ORGANISM(S): Chlamydomonas reinhardtii 
2013-01-10 | E-GEOD-40031 | biostudies-arrayexpress
TranscriptomicsThe Path to Triacylglyceride Obesity in the sta6 Strain of Chlamydomonas reinhardtii
When the sta6 (starch-null) strain of the green microalga Chlamydomonas reinhardtii is nitrogen-starved in acetate and then "boosted" after two days with additional acetate, the cells become "obese" after eight days, with triacylglyceride-filled lipid bodies filling their cytopla...
ORGANISM(S): Chlamydomonas reinhardtii 
2014-03-09 | E-GEOD-55253 | biostudies-arrayexpress
TranscriptomicsChanges in membrane lipid composition during Iron depletion and starvation in Chlamydomonas reinhardtii
C. reinhardtii cells exposed to abiotic stresses (e.g. iron-, nitrogen-, zinc- or phosphorus-deficiency) accumulate TAGs which are stored in lipid droplets. Here, we report that iron starvation leads to formation of lipids bodies and accumulation of TAGs. This occurs between 12 and 24 h of iron-star...
ORGANISM(S): Chlamydomonas reinhardtii 
2014-02-20 | E-GEOD-44611 | biostudies-arrayexpress
ProteomicsIron-starvation induces photosystem I antenna remodeling in green algae
Dunaliella salina and Dunaliella tertiolecta are extremophile, marine algae that can survive in very low Fe conditions. In this study, we used TMT-proteomics to compare the Fe starvation responses to the Fe replete responses. Samples were digested with trypsin, labeled with TMT 10-Plex, then analyze...
ORGANISM(S): Dunaliella Tertiolecta (ncbitaxon:3047) Dunaliella Cf. Bardawil Chpd1 (ncbitaxon:1227098) 
2024-11-20 | MSV000096479 | MassIVE
ProteomicsChlamydomonas reinhardtii responses to Fe-excess, Fe-deficiency, and Fe-limitation in either photoautotrophic or mixotrophic growth
A systems level analysis of Chlamydomonas reinhardtii grown photoautotrophically or mixotrophically with a reduced carbon source, acetate, under four different defined Fe stages of Fe-replete, Fe-deficient, Fe-limited, or Fe-excess. Samples were digested with trypsin, labeled with TMT 10-Plex, then ...
ORGANISM(S): Chlamydomonas Reinhardtii (ncbitaxon:3055) 
2024-11-20 | MSV000096475 | MassIVE
ProteomicsSystems analysis of Chromochloris zofingiensis during copper, iron, and zinc deficiencies
A systems level analysis of Chromochloris zofingiensis during copper, iron, and zinc deficiencies when grown photoautotrophically and mixotrophically in a revised TP medium with special K mineral nutrients.
ORGANISM(S): Chromochloris Zofingiensis (ncbitaxon:31302) 
2024-11-08 | MSV000096362 | MassIVE
TranscriptomicsRNAseq Analysis of Transcriptomic Response to Zn Resupply in Chlamydomonas reinhardtii
Zn-limited cells accumulate Cu in intracellular structures in a reversable manner. We used RNAseq analysis to monitor gene expression in Zn-limited cells, and during a time course (0 - 24 h) after Zn resupply. Comparison of Chlamydomonas reinhardtii gene expression when Zn deficient cells are resup...
ORGANISM(S): Chlamydomonas reinhardtii 
2014-11-01 | E-GEOD-58786 | biostudies-arrayexpress
TranscriptomicsSystems-level analysis of N-starvation induced modifications of carbon metabolism in a Chlamydomonas starchless mutant
To understand the molecular basis underlying increased triacylglycerol accumulation in starchless Chlamydomonas mutants, we undertook comparative time course transcriptomics of sta6 (CC-4348), a cw strain purported to represent the parental STA6 strain (CC-4349), and 3 independent STA6 strains (STA6...
ORGANISM(S): Chlamydomonas reinhardtii 
2013-10-24 | E-GEOD-51602 | biostudies-arrayexpress
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