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TranscriptomicsChromatin immunoprecipitation profiling of human HeLa cells after immunoprecipitation of ptb65 associated mRNAs
PTB is multifunctional RNA binding protein reported to be involved in splicing, 3' -end processing, stability and translational regulation. Sample preparation methods (Gama-Carvalho et al, 2006, submitted); Suspension HeLa cells were grown in DMEM, 10% FCS, Pen/Strep and split 1:2 the day before har...
ORGANISM(S): Homo sapiens 
2008-06-14 | E-GEOD-6021 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of HeLa cells after immunoprecipitation of U2AF65 associated mRNAs
U2AF65 is an essential splicing factor involved in the 3'splice site recognition dureing the first steps of spliceosome assembly. In addition, this protein has nucleocytoplasmic shuttling activity and the Drosophila homologue has been implicated in mRNA export. We have used microarray hybridization ...
ORGANISM(S): Homo sapiens 
2008-06-14 | E-GEOD-6022 | biostudies-arrayexpress
TranscriptomicsQuality assessment and control of tissue specific RNA-seq libraries of Drosophila transgenic RNAi models
Next-generation RNA-sequencing (RNA-seq) is rapidly emerging as the technology of choice for whole-transcriptome studies. However, RNA-seq is not a bias free technique. It requires large amounts of RNA and library preparation can introduce multiple artifacts, compounded by problems from later stages...
ORGANISM(S): Drosophila melanogaster 
2014-04-01 | E-GEOD-54724 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human Tfh, Tfr and Treg cells sorted from Tonsil, Iliac Lymph Node and PBMCs
The datasets were obtained to investigate the transcriptional profile of Tfh, Tfr and Treg cells isolated from different human tissues. The comparison between lymphoid tissues with different frequency of the most mature Tfh and Tfr cells allows the investigation of maturation as well as tissue adapt...
ORGANISM(S): Homo sapiens 
2021-04-22 | E-MTAB-10364 | biostudies-arrayexpress
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