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ProteomicsA combined gas phase separation strategy for ADP-ribosylated peptides
ADP-ribosylation (ADPr) is a posttranslational modification that is best studied using mass spectrometry. Method developments that are permissive to low inputs or baseline levels of protein ribosylation represent the next frontier in the field. High-field asymmetric waveform ion mobility spectrometr...
ORGANISM(S): Mus musculus (Mouse) 
2023-08-30 | PXD040898 | Pride
Mono-adp-ribose Gas-phase segmentation Ribosylation High field asymmetric waveform ion mobility spectrometry Gas-phase fragmentation 
ProteomicsThe Koala (Phascolarctos cinereus) Prostate: A Proteomic Perspective on Gland Segmentation
Australia’s most iconic animal, the koala (Phascolarctos cinereus), faces significant population decline from habitat loss, disease, and environmental pressures. Conservation has focused on assisted breeding technology and reproductive pathologies, yet koala reproductive biology remains poorly under...
ORGANISM(S): Phascolarctos cinereus (Koala) 
2026-01-23 | PXD067256 | Pride
Koala Proteomics Prostate 
ProteomicsAn evaluation of high-field asymmetric-waveform ion mobility spectrometry coupled to electron-transfer/higher-energy collision dissociation for ADP-ribosylation proteomics
Objective: ADP-ribosylation is a post-translational modification that plays an important role in cellular processes. Our previous work implemented multiple gas-phase separation strategies (e.g., FAIMS) and in-source CID on the quadrupole-Orbitrap (Exploris 480) to increase the yield and acceptor sit...
ORGANISM(S): Homo sapiens (Human) 
2025-12-26 | PXD068570 | Pride
ProteomicsAn evaluation of high-field asymmetric-waveform ion mobility spectrometry coupled to electron-transfer/higher-energy collision dissociation for ADP-ribosylation proteomics
Objective: ADP-ribosylation is a post-translational modification that plays an important role in cellular processes. Our previous work implemented multiple gas-phase separation strategies (e.g., FAIMS) and in-source CID on the quadrupole-Orbitrap (Exploris 480) to increase the yield and acceptor sit...
ORGANISM(S): Homo sapiens (Human) 
2026-04-13 | PXD067389 | Pride
Macrophage Proteomics Ethcd Hcd Adp-ribosylation 
ProteomicsHigh Dynamic Range peptide mass spectrometry using segmented precursor ions accumulation - Plasma
Limited sensitivity and depth of proteome sampling in experiments using Data-Dependent Acquisition (DDA) mass spectrometry are usually attributed to insufficient rate of fragmentation spectra acquisition relative to the number of co-eluting potential targets. Here we demonstrated that limited sensit...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
MSV000099211 | MassIVE
ProteomicsHigh Dynamic Range peptide mass spectrometry using segmented precursor ions accumulation - Method Benchmarking
Limited sensitivity and depth of proteome sampling in experiments using Data-Dependent Acquisition (DDA) mass spectrometry are usually attributed to insufficient rate of fragmentation spectra acquisition relative to the number of co-eluting potential targets. Here we demonstrated that limited sensit...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000099208 | MassIVE
ProteomicsHigh Dynamic Range peptide mass spectrometry using segmented precursor ions accumulation - SILAC
Limited sensitivity and depth of proteome sampling in experiments using Data-Dependent Acquisition (DDA) mass spectrometry are usually attributed to insufficient rate of fragmentation spectra acquisition relative to the number of co-eluting potential targets. Here we demonstrated that limited sensit...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000099210 | MassIVE
TranscriptomicsZebrafish developmental hypoxia
Study of hypoxia-induced differential expression during zebrafish development at gastrulation (shield) and segmentation (8-somite) stages An 8-chip study with 2 biological replicates from +/- hypoxia at 2 different stages of development. Each sample is prepared from 12-15 dechorionated, hand-selecte...
ORGANISM(S): Danio rerio 
2015-05-02 | E-GEOD-68473 | biostudies-arrayexpress
TranscriptomicsThe Transcriptome of Photo-Biological Hydrogen Production in the Green Alga Chlamydomonas reinhardtii
Algal photo-bio hydrogen production, a promising method for producing clean and renewable fuel in the form of hydrogen gas, has been studied extensively over the last few decades. In this study, microarray analyses were used to obtain a global expression profile of mRNA abundance in the green alga ...
ORGANISM(S): Chlamydomonas reinhardtii 
2010-05-18 | E-GEOD-9165 | biostudies-arrayexpress
ProteomicsA study into the ADP-ribosylome of IFN--stimulated THP-1 human macrophage-like cells identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-ribosylation
ADP-ribosylation is a post-translational modification that, until recently, has remained elusive to study at the cellular level. Previously dependent on radioactive tracers to identify ADP-ribosylation targets, several advances in mass spectrometric workflows now permit global identification of ADP-...
ORGANISM(S): Homo sapiens (Human) 
2020-01-27 | PXD011690 | Pride
Electron transfer higher-energy collision dissociation (ethcd) Proteomics Gas phase segmentation Higher-energy collision dissociation (hcd) Parallel reaction monitoring (prm) Orbitrap fusion lumos Post-translational modification (ptm) 
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