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ProteomicsShotgun proteomics of heterologously generated proteins and proteins in gel bands
We performed shotgun proteomics on the heterolously generated proteins and proteins in gel bands.
ORGANISM(S): Escherichia coli 
2026-07-29 | PXD070885 | Pride
Lc-ms/ms 
ProteomicsCBB staining showed beneficial effects on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS
Proteomic analysis by combining PAGE separation, gel slicing and slice-by-slice LC-MS/MS has been frequently reported in recent years. Since the MS analysis would provide identities and quantities of the proteins along the whole lane, visualization by dye staining could be skipped to save time and ...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-02-16 | PXD011689 | Pride
Sds-page In-gel digestion Proteomic analysis Cbb r-250 staining Whole gel slicing Quantitative lc-ms/ms Nondenaturing page 
TranscriptomicsUnraveling determinants of transcription factor binding outside the core binding site
Binding of transcription factors (TFs) to regulatory sequences is a pivotal step in the control of gene expression. Despite many advances in the characterization of sequence motifs recognized by TFs, our ability to quantitatively predict TF binding to different regulatory sequences is still limited....
ORGANISM(S): synthetic construct 
2015-03-04 | E-GEOD-66143 | biostudies-arrayexpress
ProteomicsTAP-ERK2-K52R MS analysis of 1D gel bands
MS analysis of 1D gel bands from TAP-ERK2-K52R immunoprecipitation experiments
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000088047 | MassIVE
ProteomicsIdentification of LMTK3 protein by LC/MS/MS Analysis of 1D SDS PAGE Gel Bands
Identification of LMTK3 by LC/MS/MS. A plasmid encoding for full-length LMTK3 was overexpressed in MCF-7 breast cancer cells. Following antibody detection by WB, two bands of interest were seen at around 200 kDa and 150 kDa on the blotting membrane. Colloidal Coomassie (CCBB) staining was performed ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD001004 | Pride
Silac Lmtk3 Breast cancer Integrin b1 Grb2 
ProteomicsPhos-tag SDS-PAGE followed by LC-MS/MS analysis of in vitro phosphorylated Nab2, Foxk1, and HURP
Purified GST-fused Nab2, Foxk1, and HURP were incubated with ATP in the presence or absence of His-tagged active ERK in vitro, separated by Phos-tag SDS-PAGE, and visualized by CBB staining. The most shifted regions of protein bands on the Phos-tag-containing gels were excised, digested with trypsin...
ORGANISM(S): Mus Musculus (mouse) 
2022-03-03 | PXD028837 | JPOST Repository
ProteomicsGlobupain gel bands digested with trypsin
Globupain in gel trypsin digest 14 = 5A 15 = 6A 16 = 7A 17 = 8A 18 = 9A 19 = 10A 20 = 11A
ORGANISM(S): Archaeoglobales (ncbitaxon:2231) 
2023-05-22 | MSV000092007 | MassIVE
ProteomicsIn gel trypsin digested 1 D resolved bands of freeze-dried camel milk
Demand for camel milk (CM) is increasing worldwide, due to its high nutritious value and health benefits. In this study, whole CM powders were produced by spray drying (SD) at six inlet temperatures (190°C - 250°C) and by freeze drying (FD). Physicochemical and functional properties of CM powder pro...
ORGANISM(S): Camelus dromedarius (Dromedary) (Arabian camel) 
2021-02-25 | PXD023290 | Pride
Soluble camel milk protein fraction of lyophilized camel milk 1 d gel bands in reducing condition of camel milk powder 
ProteomicsA new method for differential proteomic analysis utilizing native MS-electropherograms: a comparison between plasma and serum proteins by combining native PAGE, whole-gel slicing and quantitative LC-MS/MS
MS identification has long been used for PAGE-separated protein bands, but global and systematic quantitation utilizing MS after PAGE has remained rare and not been reported for native PAGE. Here we reported on a new method combining native PAGE, whole-gel slicing and quantitative LC-MS/MS aiming c...
ORGANISM(S): Homo sapiens (Human) 
2017-09-08 | PXD006773 | Pride
Gel slicing Quantitative lc-ms/ms Native ms-electropherogram Native 1d-page Human plasma and serum Differential proteomic analysis 
ProteomicsAnalysis of mouse hippocampus membrane proteome by gel-based LC/MS
Mouse hippocampus membrane fractions were prepared using sucrose density gradient ultracentrifugation. Membrane proteins were solubilized using three different condition; 6-ACA/Triton X-100 or ProteoExtract Native Membrane Protein Extraction Kit (Calbiochem, Cat. No. 444810) or ProteoExtract Transme...
ORGANISM(S): Mus musculus (Mouse) 
2014-08-15 | PXD000311 | Pride
Mouse hippocampus Ltq orbitrap velos pro Membrane proteins Gel-based proteomics In-gel chymotrypsin/trypsin digestion 
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