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ProteomicsCBB staining showed beneficial effects on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS
Proteomic analysis by combining PAGE separation, gel slicing and slice-by-slice LC-MS/MS has been frequently reported in recent years. Since the MS analysis would provide identities and quantities of the proteins along the whole lane, visualization by dye staining could be skipped to save time and ...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-02-16 | PXD011689 | Pride
Sds-page In-gel digestion Proteomic analysis Cbb r-250 staining Whole gel slicing Quantitative lc-ms/ms Nondenaturing page 
Proteomics1DE-MS profiling of rat cerebral cortex proteins of focal cerebral ischemia-reperfusion injury by combining SDS-PAGE, gel slicing, quantitative LC-MS/MS and profile reconstruction
A rat model of focal cerebral ischemia-reperfusion (I/R) injury was established by 2 hour-middle cerebral artery occlusion (MCAO) followed with 1, 7 and 14 days of reperfusion. Validation of the model was done by the measurement on cerebral blood flow, infarct volume, histological damage and neurol...
ORGANISM(S): Rattus Norvegicus 
2022-05-11 | PXD033793 |
ProteomicsA new method for differential proteomic analysis utilizing native MS-electropherograms: a comparison between plasma and serum proteins by combining native PAGE, whole-gel slicing and quantitative LC-MS/MS
MS identification has long been used for PAGE-separated protein bands, but global and systematic quantitation utilizing MS after PAGE has remained rare and not been reported for native PAGE. Here we reported on a new method combining native PAGE, whole-gel slicing and quantitative LC-MS/MS aiming c...
ORGANISM(S): Homo sapiens (Human) 
2017-09-08 | PXD006773 | Pride
Gel slicing Quantitative lc-ms/ms Native ms-electropherogram Native 1d-page Human plasma and serum Differential proteomic analysis 
UnknownProteomic analysis of endoscopically (endoscopic pancreatic function test) collected gastroduodenal fluid using in-gel tryptic digestion followed by LC-MS/MS.
Not available
S-EPMC3210639 | biostudies-literature
ProteomicsLC-MS/MS-Based Proteomic Analysis of a Single Human-Derived Gel Band
LC-MS/MS-Based Proteomic Analysis of a Single Human-Derived Gel Band
ORGANISM(S): Homo sapiens (Human) 
2026-06-11 | PXD074137 | Pride
Xb10683gi 
UnknownComparative Proteomic Profiling Using Two-Dimensional Gel Electrophoresis and Identification via LC-MS/MS Reveals Novel Protein Biomarkers to Identify Aggressive Subtypes of WHO Grade I Meningioma.
Not available
S-EPMC5582960 | biostudies-literature
ProteomicsIdentification of LMTK3 protein by LC/MS/MS Analysis of 1D SDS PAGE Gel Bands
Identification of LMTK3 by LC/MS/MS. A plasmid encoding for full-length LMTK3 was overexpressed in MCF-7 breast cancer cells. Following antibody detection by WB, two bands of interest were seen at around 200 kDa and 150 kDa on the blotting membrane. Colloidal Coomassie (CCBB) staining was performed ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD001004 | Pride
Silac Lmtk3 Breast cancer Integrin b1 Grb2 
UnknownA semi-virtual two dimensional gel electrophoresis: IF-ESI LC-MS/MS.
Not available
S-EPMC5587868 | biostudies-literature
ProteomicsBAC-DROP: Rapid digestion of proteome fractionated via dissolvable polyacrylamide gel electrophoresis and its application to bottom-up proteomics workflow
The GeLC-MS workflow, which combines low-cost, easy-to-use SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with liquid chromatography-mass spectrometry (LC-MS), is very popular in current bottom-up proteomics. However, GeLC-MS requires that PAGE-separated proteins undergo overnight enzymatic diges...
ORGANISM(S): Homo Sapiens Hepatitis B Virus Bos Taurus 
2021-01-24 | PXD021489 | panorama
ProteomicsMass spectrometry of in-gel digests reveals differences in amino acid sequences of high-molecular-weight glutenin subunits in spelt and emmer compared to common wheat
High-molecular-weight glutenin subunits (HMW-GS) play an important role for the baking quality of wheat. The ancient wheats emmer and spelt differ in their HMW-GS pattern compared to modern common wheat and this might be one reason for their comparatively poor baking quality. The aim of this study w...
ORGANISM(S): Triticum Dicoccoides Triticum Aestivum Triticum Spelta 
2020-01-15 | PXD016342 | panorama
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