SILAC quantitative affinity purification experiment was performed to compare the protein composition of pericentriolar satellites in the wild-type and acentrosomal (stil KO) DT40 cell lines.
Label-free quantitative affinity purification experiments were performed to identify the protein composition of pericentriolar satellites in the wild-type and two acentrosomal (stil KO; cep152 KO) DT40 cell lines.
SILAC quantitative proteomics was used to compare the whole cell proteomes of the differentially SILAC-labelled wild-type and acentrosomal (stil KO) DT40 cell lines.
To establish the molecular basis for CEP215 function in centrosome-spindle pole attachment, we employed an unbiased proteomic approach to isolat e and identify CEP215 interactors. To this end, affinity purification tags ( Gs TAP containing protein G and streptavidin-binding pr...
