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ProteomicsGFP pulldown for the RiboVD project
GFP-tagged viral peptide sequences and their controls were used to pull down interactors from human (uninfected) or green monkey (viral infected) cell lysate. After sample preparation, digestion and bottom-up LC-MS/MS analysis, the enriched proteins were identified.
ORGANISM(S): Homo sapiens (Human) Cercopithecus aethiops (Green monkey) (Grivet) 
2023-03-09 | PXD033874 | Pride
Pulldown Motif Virus Interaction Coronavirus 
ProteomicsNUP153 interactome in human infected/uninfected
GFP-tagged NUP153 and suitable controls were used to pull down interactors from human cell lysate, where the cells were uninfected/infected with TBEV/infected with LGTV. After sample preparation, digestion and bottom-up LC-MS/MS analysis, the enriched proteins were identified.
ORGANISM(S): Homo sapiens (Human) 
2026-03-10 | PXD052523 | Pride
Tbev Nup153 Lgtv 
UnknownAn intracellular nanotrap redirects proteins and organelles in live bacteria.
Not available
S-EPMC4313912 | biostudies-literature
ProteomicsA nanobody-based toolset to monitor and modify the mitochondrial GTPase Miro1
The mitochondrial outer membrane (MOM)-anchored GTPase Miro1, is a central player in mitochondrial transport and homeostasis. The dysregulation of Miro1 in amyotrophic lateral sclerosis (ALS) and Parkinson's disease (PD) suggests that Miro1 may be a potential biomarker or drug target in neuronal dis...
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD031218 | Pride
Nanobody Miro1 Interactome 
ProteomicsIdentification of novel Coronin 1A interacting proteins
Severe combined immunodeficiency 8 (IMD8) is caused by mutations in the human Coronin 1A (Coro1A). The clinical presentation of IMD8 patients is characterized by recurrent bacterial infections, suggesting an important role of Coro1A in innate immunity. To analyze the molecular mechanism of Coro1A du...
ORGANISM(S): Homo sapiens (Human) 
2017-06-29 | PXD004635 | Pride
Hl-60 cells Gfp nanotraps Maxquant Coronin 1a Co-immunoprecipitation Lc-ms/ms 
ProteomicsNanobodies as novel tools to monitor the mitochondrial fission factor Drp1
In cells, mitochondria undergo constant fusion and fission. An essential factor for fission is the mammalian dynamin-related protein 1 (Drp1). Dysregulation of Drp1 has been linked to neurodegenerative diseases including Parkinson’s as well as cardiovascular diseases and cancer. Here, we developed n...
ORGANISM(S): Homo sapiens (Human) 
2024-05-29 | PXD051737 | Pride
Pulldown Proteomics Mitochondrial fission Drp1 Nanobodies 
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