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ProteomicsComprehensive cross-linking mass spectrometry reveals molecular insights into the between peripheral and core NuRD complex subunits
Affinity purifications of CHD4 and CDK2AP2 to identify their interactors. The C-terminally tagged CHD4 has a C-terminal truncation to test whether this domain is required for the interaction with other NuRD subunits. GFP-purifications of both N- and C-terminally tagged CDK2AP2 were performed.
ORGANISM(S): Homo sapiens (Human) 
2020-12-07 | PXD017378 | Pride
Chd Cdk2ap2 Doc1r Nurd 
ProteomicsATG8 interactions in pexophagy conditions
In the project «ATG8 interactions in pexophagy conditions» anti-GFP affinity purifications (APs) of cells expressing (a) GFP as negative control, (b) GFP-GABARAP, (c) GFP-MAP1LC3B (LC3) were performed (each n=3 biol. replicates).
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD048003 | Pride
Autophagy Gabarap Affinity purification Map1lc3b Lc-ms/ms 
ProteomicsFungal COP9 signalosome assembly requires connection of two trimeric intermediates for integration of intrinsic deneddylase
The eight-subunit COP9 signalosome (CSN complex), controls the exchange of E3 ubiquitin cullin RING ligase receptors through its deneddylase activity, providing specificity to eukaryotic protein degradation. The conserved eight-subunit CSN complex is required for multicellular development of the fil...
ORGANISM(S): Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) 
2023-08-10 | PXD043205 | Pride
Cop9 signalosome Aspergillus nidulans Gfp-affinity purifications Csn complex Native complex assembly Subcomplexes 
ProteomicsThe interactome of a family of potential methyltransferases
Human methytransferase like proteins (METTL) are part of a large protein family characterized by the presence of binding domains for S-adenosyl methionine, a co-substrate in most methylation reactions. Despite the fact that members of this protein family were shown or predicted to be DNA, RNA or pro...
ORGANISM(S): Homo sapiens (Human) 
2019-05-06 | PXD011125 | Pride
Gfp affinity purifications Interaction proteomics Mass spectrometry Mettl 
Proteomics¬¬ZMYND8 co-localizes with NuRD on target genes and regulates poly-ADP-ribose dependent recruitment of GATAD2A/NuRD to sites of DNA damage
The NuRD complex is generally thought to repress transcription at both hyper- and hypomethylated regions in the genome. In addition, the complex is involved in the DNA damage response. Here, we show that ZMYND8 bridges NuRD to a number of putative DNA-binding zinc finger proteins. The ZMYND8 MYND do...
ORGANISM(S): Homo sapiens (Human) 
2016-10-10 | PXD003856 | Pride
Nurd Epigenetics Zmynd8 Silac Lfq Gfp-affinity purification 
UnknownImproved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels.
Not available
S-EPMC3605737 | biostudies-literature
ProteomicsEvaluation of the effects of sample size on affinity purification-mass spectrometry
An affinity purification experiment was carried out to evaluate the effects of sample size on the performance of AP-MS experiments. The Arp2/3 complex was affinity purified from Saccharomyces cerevisiae cells containing GFP-tagged Arc35, a core subunit of the complex, in-gel digested by trypsin, and...
ORGANISM(S): Saccharomyces Cerevisiae By4741 (ncbitaxon:1247190) 
2020-06-08 | MSV000085564 | MassIVE
TranscriptomicsTDP-43 Interactor and Motor Neuron Tagged Ribosome Affinity Purification in a Drosophila Model of ALS
We report the enrichment of mRNAs with motor neuron specific TDP-43 and tagged ribosomes in control as well as multiple models of TDP-43 induced neurodegeneration. Amyotrophic lateral sclerosis (ALS) is a genetically heterogeneous neurodegenerative disease in which 97% of patients exhibit cytoplasmi...
ORGANISM(S): Drosophila melanogaster 
2021-03-31 | GSE156222 | GEO
UnknownIdentification of Drosophila centromere associated proteins by quantitative affinity purification-mass spectrometry.
Not available
S-EPMC4536286 | biostudies-literature
ProteomicsFlavonol-mediated stabilization of efflux complexes regulates polar auxin transport
The transport of auxin controls the rate, direction and localization of plant growth and development. The course of auxin transport is defined by the polar subcellular localization of the PIN proteins, a family of auxin efflux transporters. Using blue native PAGE and quantitative mass spectrometry, ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-10-08 | PXD021543 | Pride
Arabidopsis thaliana Ap-ms Auxin transport 
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