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TranscriptomicsExpression analysis of Drosophila S2R+ cells depleted of M1BP by RNAi
Microarray analysis of gene expression profiles in control and M1BP-depleted Drosophila S2R+ cells. The results of this analyses are reported in Li, J. and D.S. Gilmour (2013) The newly identified transcription factor M1BP ad GAGA factor orchestrate distinct mechanisms of transcriptional regulation ...
ORGANISM(S): Drosophila melanogaster 
2013-08-13 | E-GEOD-46629 | biostudies-arrayexpress
GenomicsPermanganate ChIP-seq analysis of Pol II in nonheat shocked and heat shocked Drosophila S2R+ cells and nonheat shocked BG3 cells
The genomic distribution of transcriptionally engaged Pol II in control and heat shocked cells was determined by combining formaldehyde crosslinking and permanganate oxidation of transcription bubbles Cells treated in succession with formaldehyde and then permanganate, were subjected to chromatin pr...
ORGANISM(S): Drosophila melanogaster 
2013-07-02 | E-GEOD-46620 | biostudies-arrayexpress
GenomicsChIP-seq analysis of M1BP in Drosophila S2R+ cells
The genomic distribution of a novel transcription factor called M1BP was determined in Drosophila S2R+ cells Polyclonal antibody raised against M1BP was used to immunoprecipitate M1BP-DNA adducts generated by treating Drosophila cells with formaldehyde, lysing the cells, and shearing DNA by sonicati...
ORGANISM(S): Drosophila melanogaster 
2013-08-13 | E-GEOD-49842 | biostudies-arrayexpress
GenomicsThe newly identified transcription factor M1BP ad GAGA factor orchestrate distinct mechanisms of transcriptional regulation on paused genes
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Drosophila melanogaster 
2013-08-13 | E-GEOD-46630 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of Drosophila Schneider 2 cells using NELF-E and NELF-B antibodies
ChIP-chip analysis of GAGA factor and NELF in Drosophila S2 cells
ORGANISM(S): Drosophila melanogaster 
2008-04-04 | E-MEXP-1547 | biostudies-arrayexpress
GenomicsA crucial role of the ubiquitously expressed transcription factor Sp1 at early stages of hematopoietic specification [ChIP-Seq]
Mammalian development is regulated by the interplay of tissue-specific and ubiquitously expressed transcription factors, such as Sp1. Sp1 knock-out mice die in utero with multiple phenotypic aberrations, but the underlying molecular mechanism of this differentiation failure has been elusive. Here we...
ORGANISM(S): Mus musculus 
2014-06-12 | E-GEOD-52496 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of Drosophila DNA to identify nucleosomes bound by RNA polymerase II
Crosslinked chromatin was digested with Micrococcal nuclease and immunopurified for Rpb3, a subunit of RNA Polyermase II. chIP'd DNA was hybridized to Drosophila tiling arrays and nucleosomes that were bound by Pol II were mapped.
ORGANISM(S): Drosophila melanogaster 
2008-05-20 | E-MEXP-1515 | biostudies-arrayexpress
TranscriptomicsMapping of mononucleosomal DNA in wild type Drosophila
Crosslinked chromatin from wild-type flies was digested with micrococcal nuclease. Mononucleosomal DNA was gel-purified and hybridized to Affymetrix tiling arrays.
ORGANISM(S): Drosophila melanogaster 
2008-05-20 | E-MEXP-1519 | biostudies-arrayexpress
TranscriptomicsChromatin immunopreciptitation of Drosophila DNA using antibodies against RMA polymerase II CTD repeats
Crosslinked chromatin from wild type flies was sonicated into small fragments, and used in ChIP reactions using a monoclonal antibody, 8WG16, against RNA polymerase II CTD repeats. Input material was removed prior to chIP, and a control chIP was incubated with normal rabbit IgG. The Input and IPed...
ORGANISM(S): Drosophila melanogaster 
2008-05-20 | E-MEXP-1520 | biostudies-arrayexpress
GenomicsTissue specific Pol II Chip-Seq in the early fly embryo
In order to idetify paused promoters in vivo, we performed tissue specific Pol II Chip-seq using mutant embryos for the dorsal gradient. We used two population of cells, either dorsal ectoderm cells (gd7 embryos) or mesodermal cells (Toll10b) embryos. ChIP-seq for Pol II in various Drosophila embryo...
ORGANISM(S): Drosophila melanogaster 
2013-05-23 | E-GEOD-36310 | biostudies-arrayexpress
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