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We used the TriFecta TM (IDT, Integrated DNA Technologies, USA) anti-Aire siRNA sequence (GGAUUCUCUUUAAGGACUACAAUCTAGAUUGUAGUCCUUAAAGAGAAUCCUC) to silencing Aire mRNA. Confluent cultures of 3.10 mTEC cell line were transfected with 10 nM of anti-Aire siRNA using Hiperfect reagent (Qiagen) following ...
ORGANISM(S): Mus musculus 
In the yeast saccharoryces cerevisiae, RPA49 full-deletion mutants show a strong growth defect at 30°C and are unable to grow at 25°C. However, spontaneous suppressors that restore growth at 25°C are observed. We attend to identified new suppressor alleles after UV treatment. To mapped the genomic l...
ORGANISM(S): Saccharomyces cerevisiae 
One-step reprogramming of human fibroblasts into oligodendrocyte-like cells. We characterized the expression, chromatin accessibility and methylation profile of human oligodendrocyte-like cells derived using a new protocol for the direct transdifferentiation of human fibroblasts into oligodendrocyte...
ORGANISM(S): Homo sapiens 
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