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ProteomicsNative holdup (nHU) to measure binding affinities from cell extracts
Characterizing macromolecular interactions is essential for understanding cellular processes, yet nearly all methods used to detect protein interactions from cells are qualitative. Here, we introduce the native holdup (nHU) approach to quantify equilibrium binding constants and explore binding mecha...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD036024 | Pride
Mass spectrometry Protein-protein interaction Affinity constant determination 
ProteomicsQuantitative fragmentomics to map affinities of interactomes
~500,000 protein interactions have been identified in human but most binding affinities remain unknown, limiting quantitative investigation of interactome-function relationships. This gap can be filled by systematically measuring the affinities of minimal interacting protein fragments, the building ...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD027743 | Pride
Complexome Pdz Domain-motif interaction Interactome 
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