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TranscriptomicsHigh-throughput pyrosequencing of plant virus small RNAs
Plant virus infection involves the production of viral small RNAs (vsRNAs) with the potential to associate with distinct Argonaute (AGO)-containing silencing complexes and mediate diverse silencing effects on RNA and chromatin. We used multiplexed, high-throughput pyrosequencing to profile populatio...
ORGANISM(S): Melon necrotic spot virus 
2009-07-09 | E-GEOD-16996 | biostudies-arrayexpress
TranscriptomicsAnalysis of the melon (Cucumis melo) small RNAome by high-throughput pyrosequencing
Melon (Cucumis melo L.) is a commercially important fruit crop that is cultivated worldwide. The melon research community has recently benefited from the determination of a complete draft genome sequence and the development of associated genomic tools, which have allowed us to focus on small RNAs (s...
ORGANISM(S): Cucumis melo 
2011-08-03 | E-GEOD-28653 | biostudies-arrayexpress
TranscriptomicsRecessive resistance to Watermelon mosaic virus in melon is associated with a defense response as revealed by microarray analysis
Virus resistances that are recessively inherited are associated with loss-of-susceptibility resistance alleles. Resistance to Watermelon mosaic virus (WMV) of melon accession TGR-1551 is expressed as a drastic reduction of the virus titer, and is recessively inherited. In this work, viral RNA accumu...
ORGANISM(S): Cucumis melo 
2011-10-02 | E-GEOD-30111 | biostudies-arrayexpress
TranscriptomicsA cost-effective method for double stranded cDNA synthesis for microarray analysis
DNA microarrays are two-dimensional arrangements of specific probes deposited on a substrate that have been widely used in gene expression analysis by measuring mRNA accumulation. The use of this type of microarrays involves the synthesis of cDNA, which has to be double stranded (ds) if the microarr...
ORGANISM(S): Cucumis melo 
2012-03-07 | E-GEOD-30693 | biostudies-arrayexpress
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