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MetabolomicsPrediction and evaluation of the effect of pre-centrifugation sample management on the measurable untargeted LC-MS plasma metabolome

Optimal handling is the most important means to ensure adequate sample quality. We aimed to investigate whether pre-centrifugation delay time and temperature could be accurately predicted and to what extent variability induced by pre-centrifugation management can be adjusted for. We used untarget...

2021-10-13 | MTBLS2259 | MetaboLights
MetabolomicsDynamic utilization of low-molecular-weight organic substrates across a microbial growth rate gradient

AIM: Low-molecular-weight organic substances (LMWOSs) are at the nexus between micro-organisms, plant roots, detritus and the soil mineral matrix. The nominal oxidation state of carbon (NOSC) has been suggested as a potential parameter for modelling microbial uptake rates of LMWO...

2023-08-14 | MTBLS3558 | MetaboLights
TranscriptomicsFluidigm Biomark 96.96 nanofluidic arrays for qRT-PCR of 93 miRNAs: salivary extracellular vesicles
Crude extracellular vesicles (EVs) from eight healthy volunteers were separated into 6 fractions based on their densities by using the iodixanol-based density gradient centrifugation method. To determine the distribution of miRNAs among these fractions, quantities of 93 miRNAs were quantified by the...
ORGANISM(S): Homo sapiens 
2016-08-01 | E-GEOD-78763 | biostudies-arrayexpress
TranscriptomicsImmune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube and standard density gradient
High quality genetic material is an essential pre-requisite when analyzing gene expression using microarray technology. Peripheral blood mononuclear cells (PBMC) are frequently used for genomic analyses, but several factors can affect the integrity of nucleic acids prior to their extraction, includi...
ORGANISM(S): Homo sapiens 
2015-08-28 | E-GEOD-67321 | biostudies-arrayexpress
TranscriptomicsLocolization of circRNA with DIS3 WT or D146N mutant in sucrose density gradient centrifugation assay
To investigate the locolization between DIS3 and circRNA in fractions form sucrose density gradient centrifugation (SDGC) assay for 293T cells
ORGANISM(S): Homo sapiens 
2025-02-16 | GSE256498 | GEO
GenomicsLocolization of circRNA with DIS3 WT or D146N mutant in sucrose density gradient centrifugation assay
Locolization of circRNA with DIS3 WT or D146N mutant in sucrose density gradient centrifugation assay
PRJNA1079793 | ENA
TranscriptomicsSubpopulation array data, Veening et al.
The transcriptome profiles of sporulating vs non-sporulating cells, within an isogenic culture were compared. RNA was isolated from endospore containing cells which were separated from non-spore forming cells using buoyant density gradient centrifugation within an isogenic culture (details in Veenin...
ORGANISM(S): Bacillus subtilis 
2010-05-25 | E-GEOD-9266 | biostudies-arrayexpress
ProteomicsProteomic Analysis of the Protein Composition of Migrasomes Enriched by Magnetic Aptamer Probe System and Density Gradient Centrifugation
This study aims to evaluate the performance of the magnetic aptamer probe (MAP) system in the enrichment of migrasomes. First, the MAP system was constructed by functionalizing biotin-labeled migrasome-targeting aptamers (Apt_B3) onto the surface of NeutrAvidin-coated magnetic sub-micrometre particl...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD074521 | Pride
Density gradient centrifugation Proteomic Aptamer Migrasome 
UnknownSingle-cell mass-density measurements using microchannel gradient centrifugation.
Not available
S-EPMC12909970 | biostudies-literature
TranscriptomicsSingle-cell RNA sequencing of PBMCs from five healthy (HC) preterm infants and ten preterm infants with bronchopulmonary dysplasia(BPD)
We procured PBMCs whole blood from five HC preterm infants and five preterm infants with BPD. PBMCs were extracted using a density gradient centrifugation method. Initially, 10ml of peripheral blood was mixed with an equal volume of physiological saline, then carefully layered onto Ficoll solution (...
ORGANISM(S): Homo sapiens 
2024-10-20 | E-MTAB-14507 | biostudies-arrayexpress
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